Some Rheological Properties of Perfluorocarbon Emulsions and Their Potential use in Countercurrent Chromatography Distribution and Chromatography Separation Systems

  • Ian A. Sutherland
  • John Eveleigh


Aqueous two phase systems utilising polymers such as dextran and poly(ethylene) glycol have offered a unique method of purifying viable biological material for more than three decades. Perhaps the most inhibiting factors limiting their widespread use and application today is their cost, poor physical characteristics and variable reproducibility. New cheaper polymers are now coming on the market but their high viscosity, low interfacial tension and low density difference still result in long settling times which make high resolution countercurrent distribution laborious and continuous countercurrent extraction almost impossible.


Phase System Countercurrent Distribution Emulsion Phase Countercurrent Chromatography Application Today 
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  1. 1.
    J. Eveleigh, Immiscible liquid affinity supports, in: “Separations Using Aqueous Phase Systems: Applications in Cell Biology and Biotechnology,” D. Fisher and I.A. Sutherland, eds., Plenum, New York (1989)Google Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Ian A. Sutherland
    • 1
  • John Eveleigh
    • 2
  1. 1.Engineering DepartmentNational Institute for Medical ResearchLondonUK
  2. 2.Department of Medical ProductsE.I. du Pont de Nemours & Co IncWilmingtonUSA

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