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Protein Partitioning Between Microemulsions and Conjugate Aqueous Phases

  • P. D. I. Fletcher
  • D. Parrott

Abstract

Water-in-oil (W/O) microemulsions consist of thermodynamically-stable dispersions of tiny water droplets (radii 1–20 nm) in oil stabilised by a surfactant. These phases can co-exist with aqueous phases at equilibrium. Added proteins partition between the two phases to a degree which is sensitive to droplet size, aqueous phase salt concentration and protein charge. Hence these systems offer potential for the selective extraction of proteins. Furthermore, partitioning results for enzymes and substrates are useful in interpreting changes in enzyme catalytic activity in microemulsions as compared with bulk water.

Keywords

Partition Coefficient Droplet Size Water Droplet Bulk Water Faraday Trans 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • P. D. I. Fletcher
    • 1
  • D. Parrott
    • 1
  1. 1.School of ChemistryThe University of HullHull, North HumbersideUK

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