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Affinity Partitioning of Phosphofructokinase from Rat Erythrocytes in Poly(Ethylene Glycol)-Cibacron Blue/Dextran Two-Phase Systems

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Separations Using Aqueous Phase Systems

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Abstract

Partition of enzymes between two liquid aqueous phases can be highly influenced by specific ligands bound to a water-soluble polymer included in the two-phase system. The fact that phosphofructokinase (PFK) binds strongly Cibacron blue (Cb) allow the use of such a ligand to study Cibacron blue-enzyme interaction [1–4] as well as to obtain PFK-enriched fractions from crude extracts [1].

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References

  1. G. Kopperschläger and G. Johansson, Anal. Biochem. 124:117 (1982)

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Author information

Authors and Affiliations

  1. Departamento de Bioquimica y Biologia Molecular, Universidad de Alcalá de Henares, Alcalá de Henares (Madrid), Spain

    M. C. Tejedor, M. Grupelli, A. Rubio, C. Delgado & J. Luque

Authors
  1. M. C. Tejedor
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  2. M. Grupelli
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  3. A. Rubio
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  4. C. Delgado
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  5. J. Luque
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Editor information

Editors and Affiliations

  1. Royal Free Hospital School of Medicine, London, UK

    Derek Fisher

  2. National Institute for Medical Research, London, UK

    Ian A. Sutherland

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© 1989 Plenum Press, New York

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Tejedor, M.C., Grupelli, M., Rubio, A., Delgado, C., Luque, J. (1989). Affinity Partitioning of Phosphofructokinase from Rat Erythrocytes in Poly(Ethylene Glycol)-Cibacron Blue/Dextran Two-Phase Systems. In: Fisher, D., Sutherland, I.A. (eds) Separations Using Aqueous Phase Systems. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5667-7_7

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  • DOI: https://doi.org/10.1007/978-1-4684-5667-7_7

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5669-1

  • Online ISBN: 978-1-4684-5667-7

  • eBook Packages: Springer Book Archive

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