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Affinity Partitioning of Phosphofructokinase from Rat Erythrocytes in Poly(Ethylene Glycol)-Cibacron Blue/Dextran Two-Phase Systems

  • M. C. Tejedor
  • M. Grupelli
  • A. Rubio
  • C. Delgado
  • J. Luque

Abstract

Partition of enzymes between two liquid aqueous phases can be highly influenced by specific ligands bound to a water-soluble polymer included in the two-phase system. The fact that phosphofructokinase (PFK) binds strongly Cibacron blue (Cb) allow the use of such a ligand to study Cibacron blue-enzyme interaction [1–4] as well as to obtain PFK-enriched fractions from crude extracts [1].

Keywords

Ionic Strength Partition Coefficient Polymer Concentration High Salt Concentration Cibacron Blue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • M. C. Tejedor
    • 1
  • M. Grupelli
    • 1
  • A. Rubio
    • 1
  • C. Delgado
    • 1
  • J. Luque
    • 1
  1. 1.Departamento de Bioquimica y Biologia MolecularUniversidad de Alcalá de HenaresAlcalá de Henares (Madrid)Spain

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