Quantitation of the Resolving Power of CCD using Human Erythrocytes
Although CCD has been applied to the separation and analysis of a number of different cell types, there has been little attempt to quantitate the resolving power of this technique. In this study, the resolving power of CCD has been examined using neuraminidase-treated and untreated human erythrocytes as model cells. By treating erythrocytes with different concentrations of neuraminidase, cell populations differing in their sialic acid content, and hence their surface negative charge, were obtained. The ability of CCD to resolve neuraminidase-treated and control erythrocytes, exhibiting increasingly similar amounts of surface sialic acid, was determined using 30-transfer CCD and a phase system of high electrostatic potential difference between the phases (ΔΨ). The limit of resolution of CCD was expressed in terms of the μg difference in sialic acid content between the two red cell populations which were just resolved.