A New Approach to Countercurrent Distribution Combining Separation with Analysis in the Biomek Automated Laboratory Workstation
A method of programming the Biomek Automated Laboratory Workstation has been developed to perform countercurrent distribution separations of both solutes and cells using aqueous two-phase systems. Studies involving an 11-transfer CCD show good correlation between experimental and theoretical distributions. The separation of high and low partition coefficient cells have shown that the Biomek’s mixing and transfer regimes work well for both particulates and solubles. These results could form the basis of a new approach to the separation of viable biological material and extend the range of an already versatile piece of laboratory equipment.
KeywordsPhase System Distribution Profile Settling Time Countercurrent Distribution Countercurrent Chromatography
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