Column Based Liquid/Liquid Separation of Cells Using Aqueous Polymeric Two-Phase Systems

  • D. R. Skuse
  • D. E. Brooks


Column-based aqueous polymeric two-phase systems were used to effect separation of dog and human erythrocytes on the basis of their interactions with a PEG-oleate affinity ligand. The resolution obtained was substantially higher than would be expected in a corresponding single tube experiment. Cell recoveries were also very high. However, elution volumes were independent of PEG-oleate concentration indicating that multiple partition steps, analagous to CCD, did not occur.


Human Erythrocyte Phase System Elution Profile Cell Recovery Dibasic Sodium Phosphate 


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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • D. R. Skuse
    • 1
  • D. E. Brooks
    • 1
  1. 1.Departments of Chemistry and PathologyUniversity of British ColumbiaVancouverCanada

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