Column Based Liquid/Liquid Separation of Cells Using Aqueous Polymeric Two-Phase Systems
Column-based aqueous polymeric two-phase systems were used to effect separation of dog and human erythrocytes on the basis of their interactions with a PEG-oleate affinity ligand. The resolution obtained was substantially higher than would be expected in a corresponding single tube experiment. Cell recoveries were also very high. However, elution volumes were independent of PEG-oleate concentration indicating that multiple partition steps, analagous to CCD, did not occur.
KeywordsHuman Erythrocyte Phase System Elution Profile Cell Recovery Dibasic Sodium Phosphate
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