Application of Liquid-Liquid Partition Chromatography (LLPC) in the Preparation of Steroid Binding Proteins

  • Arnulf Heubner
  • Michael Juchem
  • Werner Müller
  • Kunhard Pollow


Two human serum proteins, i.e. sex hormone binding globulin (h-SHBG) and corticosteroid binding globulin (h-CBG), rat corticosteroid binding globulin (r-CBG), and progesterone binding globulin (PBG) from new guinea pig were purified by the application of three different modes of chromatography. The proteins were purified by affinity chromatography and anion exchange chromatography. Fractions containing the steroid binding proteins were finally purified by liquid-liquid partition chromatography on LiParGel 750 (Merck, Darmstadt, FRG). This Chromatographic sequence clearly separated the steroid binding proteins from other proteins, mainly from serum albumin without a loss of protein and completely retaining the binding affinity towards steroids.


Anion Exchange Chromatography Corticosteroid Binding Globulin Steroid Binding Protein Crude Serum Aminopentanoic Acid 
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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Arnulf Heubner
    • 1
  • Michael Juchem
    • 1
  • Werner Müller
    • 2
  • Kunhard Pollow
    • 1
  1. 1.Department of Experimental EndocrinologyJohannes Gutenberg University MainzMainzGermany
  2. 2.E. Merck, Forschung ReagenzienDarmstadtGermany

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