Liquid-Liquid Partition Chromatography of Biopolymers in Aqueous Two-Phase Polymer Systems
The classical concept of liquid-liquid partition chromatography (LLPC) presented by Martin & Synge in 1941 may be successfully applied for Chromatographic separations of biopolymers when aqueous-aqueous two-phase polymer systems, introduced for the partition of biopolymers and cells by P.-Å. Albertsson in 1958 are used. The problem of immobilising one of the two aqueous phases selectively to a support could be solved by exploiting the incompatibility between a polymer grafted on the support surface and one of the polymers forming the two-phase system. For Albertsson’s two-phase system, based on poly(ethylene glycol) (“PEG”) and dextran (“Dx”), a support coated with polyacrylamide repels the PEG-rich phase since polyacrylamide and PEG turned out to be incompatible. This repulsion causes, on the other hand, a binding of the Dx-rich phase to the support.
KeywordsHuman Serum Albumin Partition Coefficient Total Ionic Strength Sample Constituent Linear Polyacrylamide
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