Integration of Bioconversion and Down-Stream Processing — Starch Hydrolysis in an Aqueous Two-Phase System
Starch hydrolysis is one of the major processes catalysed by enzymes in industrial operation today. Since it deals with the enzymatic degradation of a macromolecular or even particulate substrate, the use of soluble enzymes has been preferred. Since the enzymes used are rather cheap, it has been possible to operate the process under economically acceptable conditions, in spite of the consumption of enzymes. However, the reaction time is long because the biocatalytic density is kept rather low, since the soluble enzymes are not reused.