Advertisement

Process Simulation of Continuous Bioconversions in Aqueous Two-Phase Systems

  • Christian Reisinger
  • Michael Narodoslawsky
  • Anton Moser

Abstract

Progress in biotechnology largely depends on fermentation optimisation and improvement in downstream processing. These improvements get strongly supported if an integration of fermentation and product extraction can be verified in one processing unit. Continuous bioconversions in aqueous two-phase systems show a great potential of combining these two unit operations. General requirements are the capability of cells and substrates to enrich in one of the phases and the tendency of products to diffuse in the opposite phase. This integrated process is most promising for reaction systems, which exhibit kinetics characterised by product inhibition and, in addition, the process facilitates cell recycling and supports an increase in cell concentration. However, the industrial realisation of this technique is still not fully developed mainly due to difficulties in measurement and control, and to a lack of profitable products. In this work the conversion of glucose to ethanol is used as a model study to develop mathematical optimisation criteria and to create a “feasibility window” for the ongoing search for products.

Keywords

Specific Growth Rate Dilution Rate Product Inhibition Bottom Phase Hydraulic Residence Time 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    P.-Å. Albertsson, Partition of Cell Particles and Macromolecules, 3rd ed., John Wiley & Sons (1985)Google Scholar
  2. 2.
    M.-R. Kula, Liquid-liquid extraction of biopolymers, in: “Comprehensive Biotechnology,” Vol. 2, Pergamon Press (1985)Google Scholar
  3. 3.
    B. Mattiasson, Applications of aqueous two-phase systems in biotechnology, Trends in Biotech. 1:16 (1983)CrossRefGoogle Scholar
  4. 4.
    B. Mattiasson and M. Larsson, Extractive bioconversion with emphasis on solvent production, Biotech. and Gen. Eng. Reviews 3:137 (1985)Google Scholar
  5. 5.
    M. Larsson and B. Mattiasson, Novel process technology for biotechnological solvent production, Chem. and Ind. June (1984)Google Scholar
  6. 6.
    C.W. Lee and H.N. Chang, Kinetics of ethanol fermentations in membrane cell recycle fermentors, Biotech. Bioeng. 23:1105 (1987)CrossRefGoogle Scholar
  7. 7.
    A. Moser, Bioprozesstechnik, Springer-Verlag, Berlin (1981)Google Scholar
  8. 8.
    N.W.F. Kossen and N.M.G. Oosterhuis, Modelling and scaling-up of bioreactors, in: “Biotechnology,” Vol. 2, Verlang Chemie, (1985)Google Scholar
  9. 9.
    B.L. Maiorella et al, Economic evaluation of alternative ethanol fermentation processes, Biotech. Bioeng. 26:1003 (1984)CrossRefGoogle Scholar
  10. 10.
    B. Hahn-Hägerdal, Fermentation and product formation in aqueous two-phase systems with yeast and bacillus Sp., Lectures given at a Workshop of the WP Microbial Physiology of the EFB, University of Lund (1986)Google Scholar
  11. 11.
    T.K. Ghose and R.D. Tyagi, Rapid ethanol fermentation of cellulose hydrolysate II. Product and substrate inhibition and optimisation fermentor design, Biotech. Bioeng. 21:1401 (1979)CrossRefGoogle Scholar
  12. 12.
    C. D. Bazua and C.R. Wilke, Ethanol effects on the kinetics of a continuous fermentation with Sacch. cer., Biotech. Bioeng. Symp. 7:105 (1977)Google Scholar
  13. 13.
    G.K. Hoppe and G.S. Hansford, Ethanol inhibition of continuous anaerobic yeast growth, Biotech. Letters 4:39 (1982)CrossRefGoogle Scholar
  14. 14.
    S. Aiba et al, Kinetics of product inhibition in alcohol fermentation, Biotech. Bioeng. 10:845 (1968)CrossRefGoogle Scholar
  15. 15.
    G.R. Cysewski and C.R. Wilke, Rapid ethanol fermentations using vacuum and cell recycle, Biotech. Bioeng. 19:1125 (1977)CrossRefGoogle Scholar
  16. 16.
    F.F. Pironti, Kinetics of alcohol fermentations, Ph.D. Thesis, Cornell UniversityGoogle Scholar
  17. 17.
    F. Kollerup and A.J. Daugulis, A mathematical model for ethanol production by extractive fermentation in CST fermentor, Biotech. Bioeng. 27:583 (1985)CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Christian Reisinger
    • 1
  • Michael Narodoslawsky
    • 2
  • Anton Moser
    • 1
  1. 1.Institute of Biotechnology Microbiology and Waste TechnologyGrazAustria
  2. 2.Institute of Chemical EngineeringGrazAustria

Personalised recommendations