Gravity Settling of APS — Characteristics and Process Applications

  • K. H. Kroner
  • H. Hustedt


Aqueous two-phase systems (APS) have been described as useful for the extractive purification of enzymes in large scale [1] and also for applications in bioconversion processes [2]. Albertsson [3] has described these systems and their use in biochemical research basically. At large-scale processing phase separation is conducted either by using common centrifugal separators or by settling under unit gravity. This paper analyses the settling behaviour of model systems with regard to their physicochemical properties; further the application of settling in extractive enzyme recovery processes is considered.


Interfacial Tension Settling Behaviour Bioconversion Process Processing Phase Separation Unit Gravity 
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  1. 1.
    M.-R. Kula, K.H. Kroner and H. Hustedt, Purification of enzymes by liquid-liquid extraction, in: “Advances in Biochemical Engineering,” A. Fiechter, ed., Springer, Berlin (1982)Google Scholar
  2. 2.
    B. Mattiasson, M. Larsson, Bioconversion in aqueous two-phase systems, in: “Third Europ. Congress on Biotechnol.,” Verlag Chemie, Weinheim (1984)Google Scholar
  3. 3.
    P.-A. Ålbertsson, Partition of cell particles and macromolecules, Wiley Interscience, New York (1971)Google Scholar
  4. 4.
    H. Hustedt, K.H. Kroner, M.-R. Kula, Applications of phase partitioning in biotechnology, in: “Partitioning in Aqueous Two-Phase Systems — Theory, Methods, Uses and Applications to Biotechnology,” H. Walter, D.E. Brooks, D. Fisher, eds., Academic Press, New York (1985)Google Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • K. H. Kroner
    • 1
  • H. Hustedt
    • 1
  1. 1.GBF Gesellschaft für Biotechnologische Forschung mbHBraunschweigGermany

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