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Fast Downstream Processing of Proteins by Flocculation and Liquid Extraction

  • H. Hustedt
  • R. Krützfeldt
  • N. Papamichael

Abstract

The initial downstream processing of intracellular microbial enzymes is usually conducted in the sequence: cell harvesting→cell disruption→ clarification→ initial enrichment. The efficiency of the conventional recovery route — most importantly the space-time yield, but also the activity yield — can often be increased considerably by using flocculation, mechanical cell disruption and liquid extraction. A further enhancement of the space-time yield or reduction in process time, respectively, may be obtained by the integration of single steps to semi or fully continuous processes. These topics are discussed in this paper using the downstream processing of fumarase from Brevibacterium ammoniagenes as a case example based on experiments conducted in pilot/commercial scale.

Keywords

Liquid Extraction Cell Disruption Downstream Processing High Pressure Homogenisation Bead Mill 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    H. Hustedt, N. Papamichael, K.H. Kroner, R. Krützfeldt, “Synthesis of efficient downstream processing lines for proteins,” in: “Biotechnology in Germany 1987,” Dechema, Frankfurt (1987)Google Scholar
  2. 2.
    H. Hustedt, K.H. Kroner and N. Papamichael, in: “Separations Using Aqueous Phase Systems: Applications in Cell Biology and Biotechnology,” D. Fisher and I.A. Sutherland, eds., Plenum, New York (1989)Google Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • H. Hustedt
    • 1
  • R. Krützfeldt
    • 1
  • N. Papamichael
    • 1
  1. 1.GBF, Gesellschaft für Biotechnologische Forschung mbHBraunschweigGermany

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