Continuous Affinity Partitioning in a Poly(Ethylene Glycol)-Reppal Pes Aqueous Two-Phase System

  • Shaun P. Foaden
  • Howard A. Chase
  • Christopher R. Lowe


The starch-based polymer, Reppal Pes, has been used to form two-phase affinity systems with dyed-poly(ethylene glycol). Lactate dehydrogenase has been purified from a crude rabbit muscle extract in these systems using the triazine dyes Cibacron blue F3G-A and Procion Orange H-ER bound to poly(ethylene glycol). Processing parameters have been optimised in small-scale batch experiments and the results used to formulate systems for continuous affinity partitioning of rabbit muscle lactate dehydrogenase in a simple two-stage mixer-settler unit. Polymer-bound ligand from the eluting stage has been recycled into the affinity partitioning stage, thereby reducing the amount of immobilised ligand required.


Product Stream Bottom Phase Settling Chamber Pyruvate Kinase Activity Purification Ratio 
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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Shaun P. Foaden
    • 1
  • Howard A. Chase
    • 1
  • Christopher R. Lowe
    • 2
  1. 1.Department of Chemical EngineeringUniversity of CambridgeCambridgeUK
  2. 2.Institute of BiotechnologyUniversity of CambridgeCambridgeUK

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