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Aqueous Two-Phase Partition in Biochemical Recovery from Mammalian Cell Culture

  • Jon G. Huddleston
  • Andrew Lyddiatt

Abstract

A study of the fractionation of monoclonal antibodies and other solutes in spent mammalian cell culture media has been undertaken with aqueous PEG-phosphate partition systems. Manipulation of pH and average molecular weight of polymers yielded systems capable of separating transferrin from other components. Introduction into dispersed phases of a mixture of anti-BSA Sepharose and human-IgG silica promoted selective recovery of BSA, anti-huIgG monoclonal antibody and transferrin in a “four phase”, batch system distinguished by the discrete separation of antibody product and deleterious proteolytic activity into separate aqueous phases.

Keywords

Batch System Acidic Protease Bottom Phase Retention Coefficient Partition System 
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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Jon G. Huddleston
    • 1
  • Andrew Lyddiatt
    • 1
  1. 1.Biochemical Recovery Laboratory, Department of Chemical EngineeringUniversity of BirminghamBirminghamUK

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