Aqueous Two-Phase Partition in Biochemical Recovery from Mammalian Cell Culture
A study of the fractionation of monoclonal antibodies and other solutes in spent mammalian cell culture media has been undertaken with aqueous PEG-phosphate partition systems. Manipulation of pH and average molecular weight of polymers yielded systems capable of separating transferrin from other components. Introduction into dispersed phases of a mixture of anti-BSA Sepharose and human-IgG silica promoted selective recovery of BSA, anti-huIgG monoclonal antibody and transferrin in a “four phase”, batch system distinguished by the discrete separation of antibody product and deleterious proteolytic activity into separate aqueous phases.
KeywordsBatch System Acidic Protease Bottom Phase Retention Coefficient Partition System
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