Continuous Crosscurrent Extraction of Proteins in Process Scale

  • Helmut Hustedt
  • Karl-Heinz Kroner
  • Neophytos Papamichael


Automated continuous cross-current extraction of intracellular microbial enzymes from disrupted cells has been studied using a two-stage plant capable of processing 20–30 kg biomass per hour. The results reveal the feasibility of this advanced method of downstream processing by aqueous two-phase extraction.


Bottom Phase Penicillin Acylase Crosscurrent Extraction Aqueous Phase System Continuous Crosscurrent Extraction 
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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Helmut Hustedt
    • 1
  • Karl-Heinz Kroner
    • 1
  • Neophytos Papamichael
    • 1
  1. 1.GBF, Gesellschaft für Biotechnologische Forschung mbHBraunschweigGermany

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