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Mechanism of Cell Partitioning; Equilibrium Contact Angle Measurements Predict Relative, Kinetic Partitioning Behaviour

  • Bernadette N. Youens
  • W. David Cooper
  • Frank D. Raymond
  • Paul S. Gascoine
  • Derek Fisher

Abstract

The relationships between cell partition, a dynamic process, and contact angle determinations, which are equilibrium thermodynamic measurements are investigated experimentally in biphasic systems of dextran and poly(ethylene glycol). Non-charge-sensitive partition, charge-sensitive partition and hydrophobic affinity cell partition all show correlations with contact angle measurements. Contact angles appear to provide a quantitative index of the degree of interaction of cells with the liquid-liquid interfaces of phase droplets which determine the kinetic features of partitioning.

Keywords

Contact Angle Contact Angle Measurement Phase System Partition Ratio Countercurrent Distribution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Bernadette N. Youens
    • 1
  • W. David Cooper
    • 2
  • Frank D. Raymond
    • 1
  • Paul S. Gascoine
    • 1
  • Derek Fisher
    • 1
  1. 1.Department of Biochemistry, Royal Free Hospital School of MedicineUniversity of LondonLondonUK
  2. 2.Shell Research LtdThornton Research CentreChesterUK

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