Macrophage Surface Affinity Changes During Phagocytosis

  • Samuel Schürch
  • Nigel A. M. Paterson
  • Donald J. L. McIver

Abstract

We have used a combination of surface thermodynamic, spectro-photometric and morphometric techniques to investigate the mechanism of particle ingestion (phagocytosis) by isolated alveolar macrophages. We studied the time and dose-dependence of the effects on macrophages of both structurally specific particles (serum complement-opsonised zymosan) and non-specific particles (non-opsonised silica). Particle exposure increased the cell affinity for the dextran phase of 4% Dextran 2M/4% PEG 20K phases in a dose-and opsonin-dependent manner which correlated well with biochemical and morphological assessment of cell activation. We do not yet know the structural basis of the cell surface changes which are responsible for the alterations in polymer phase affinity, but likely candidates are phagocytosis-induced changes in the amount, conformation or molecular weight of membrane glycoproteins.

Keywords

Hydrated Silican Hydrocarbon Superoxide Lime 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    W.O. Fenn, The theoretical response of living cells to contact with solid bodies, J. Gen. Physiol. 4:373 (1922)PubMedCrossRefGoogle Scholar
  2. 2.
    C.J. Van Os, C.F. Gilman and A.W. Neumann, in: “Phagocytic Engulf ment and Cell Adhesiveness as Surface Phenomena,” Marcel Dekker, New York (1975)Google Scholar
  3. 3.
    P.J. Edelson, Monocytes and macrophages: aspects of their cell biology, in: “The Cell Biology of Inflammation,” G. Weissmann, ed., Elsevier, New York (1980)Google Scholar
  4. 4.
    J. Aggeler and Z. Werb, Ultrastructural aspects of phagocytosis by macrophages, in: “Advances in Inflammation Research”, Vol. 8,″ G. Weissmann, ed., Raven, New York (1984)Google Scholar
  5. 5.
    S. Schürch, D.F. Gerson and D.J.L. McIver, Determination of cell-medium interfacial tensions from contact angles in aqueous polymer systems, Biochim. Biophys. Acta 640:557 (1981)PubMedCrossRefGoogle Scholar
  6. 6.
    D.J.L. McIver and S. Schürch, Interfacial free energies of intact and reconstituted erythrocyte surfaces; implications for biological adhesion, Biochem. Biophys. Acta 691:52 (1983)Google Scholar
  7. 7.
    N.A.M. Paterson, D.J.L. McIver and S. Schürch, Zymosan enhances leukotriene D4 metabolism by porcine alveolar macrophages, Immunology 56:153 (1985)PubMedGoogle Scholar
  8. 8.
    A.W. Neumann, Contact angles and their temperature dependence: Thermodynamic status, measurement, interpretation and application, Adv. Colloid Interface Sci. 4:105 (1974)CrossRefGoogle Scholar
  9. 9.
    D.E. Brooks, K.A. Sharp and D. Fisher, Theoretical aspects of partitioning, in: “Partitioning in Aqueous Two-Phase Systems. Theory, Methods, Uses and Applications to Biotechnology,” H. Walter, D.E. Brooks and D. Fisher, eds., Academic Press, Orlando (1985)Google Scholar
  10. 10.
    E.A. Evans, Intrinsic colloidal attraction/repulsion between lipid bilayers and strong attraction induced by non-adsorbing polymers, International Symposium on Molecular Mechanisms of Membrane Fusion. Buffalo, N.Y. (1987)Google Scholar
  11. 11.
    P.J. Flory, Thermodynamics of high polymer solutions. J. Chem. Phys. 9:660 (1941)CrossRefGoogle Scholar
  12. 12.
    S. Schürch, K. Ellis and D.J.L. McIver, 14C-labelled protein adsorption at a lipid-water interface investigated by using phase separated aqueous polymers. American Chemical Society. 57th Colloid and Surface Symposium. Toronto, A 217 (1983)Google Scholar
  13. 13.
    S.A. Johnstone, S. Schürch, D.J.L. McIver, E.A. Jacobson and E.R. Tustanoff, Membrane glycoprotein and surface free energy changes in hypoxic fibroblast cells, Biochim. Biophys. Acta 815:159 (1985)PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Samuel Schürch
    • 1
  • Nigel A. M. Paterson
    • 2
    • 3
  • Donald J. L. McIver
    • 3
    • 4
  1. 1.Faculty of MedicineUniversity of CalgaryCalgaryCanada
  2. 2.Departments of MedicineUniversity of Western OntarioLondonCanada
  3. 3.Departments of Pharmacology and ToxicologyUniversity of Western OntarioLondonCanada
  4. 4.Departments of Medical BiophysicsUniversity of Western OntarioLondonCanada

Personalised recommendations