Protein Transport Processes in the Water-Water Interface in Incompatible Two Phase Systems
Proteins, such as ovalbumin, albumin, cytochrome C or a chromobacter lipase were added to either the upper or lower phase of a PEG 6000, Dextran T500 system, and the phases placed carefully in contact. Diffusion processes and interfacial accumulation were then observed by using the optical scanning system of an MSE Centriscan ultracentrifuge run at low speeds. The overall process was one of simple diffusion, though in such complex systems movement up the protein concentration gradient can occur and was observed. Interfacial accumulation roughly in accord with expectation based on a simple interfacial tension theory was also seen.
KeywordsHyaluronic Acid Lower Phase Hydrostatic Pressure Difference Interfacial Accumulation Aqueous Phase System
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