Protein Transport Processes in the Water-Water Interface in Incompatible Two Phase Systems

  • M. P. Tombs
  • S. E. Harding


Proteins, such as ovalbumin, albumin, cytochrome C or a chromobacter lipase were added to either the upper or lower phase of a PEG 6000, Dextran T500 system, and the phases placed carefully in contact. Diffusion processes and interfacial accumulation were then observed by using the optical scanning system of an MSE Centriscan ultracentrifuge run at low speeds. The overall process was one of simple diffusion, though in such complex systems movement up the protein concentration gradient can occur and was observed. Interfacial accumulation roughly in accord with expectation based on a simple interfacial tension theory was also seen.


Hyaluronic Acid Lower Phase Hydrostatic Pressure Difference Interfacial Accumulation Aqueous Phase System 
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  1. 1.
    H. Tompa, Polymer solutions, Butterworths, London (1956)Google Scholar
  2. 2.
    E. Edmond and A.G. Ogston, Biochem. J. 109:569 (1968)PubMedGoogle Scholar
  3. 3.
    A.G. Ogston, B.N. Preston and J.D. Wells, Proc. Roy. Soc. A. 333:297 (1973)CrossRefGoogle Scholar
  4. 4.
    T.C. Laurent, Chemistry and physiology of polysaccharides, Proc. Milan Symp. Churchill, London (1968)Google Scholar
  5. 5.
    A.G. Ogston, J. Phys. Chem. 74:668 (1970)CrossRefGoogle Scholar
  6. 6.
    J.D. Wells, J. Phys. Chem. 90:2433 (1968)CrossRefGoogle Scholar
  7. 7.
    T.C. Laurent, B.N. Preston and L.O. Sundeloff, Nature 279:60 (1979)PubMedCrossRefGoogle Scholar
  8. 8.
    P.-Å. Albertsson, Partition of Cell Particles and Macromolecules, 3rd edition, Wiley, New York (1985)Google Scholar
  9. 9.
    M.P. Tombs, B. Newsom and P. Wilding, Int. J. Prot. and Peptide Res. 6:253 (1974)CrossRefGoogle Scholar
  10. 10.
    J. England and G.M. Nazarian, Biochemistry 9:1553 (1970)PubMedCrossRefGoogle Scholar
  11. 11.
    V.P. Shanbhag, Biochem. Biophys. Acta 320:517 (1973)PubMedCrossRefGoogle Scholar
  12. 12.
    P. Meares, Farad. Discussions Chem. Soc. 77:7 (1984)CrossRefGoogle Scholar
  13. 13.
    H. Linde, Farad. Discussions Chem. Soc. 77:181 (1984)CrossRefGoogle Scholar
  14. 14.
    T.C. Laurent and B. Preston, J. Phys. Chem. 87:648 (1983)CrossRefGoogle Scholar
  15. 15.
    J.D. Wells, K. Edsman, T.C. Laurent and L.O. Sundeloff, J. Phys. Chem. 90:2425 (1986)CrossRefGoogle Scholar
  16. 16.
    G.A. Roberts and M.P. Tombs, Biochem. Biophys. Acta 902:327 (1987)PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • M. P. Tombs
    • 1
  • S. E. Harding
    • 1
  1. 1.Department of Applied Biochemistry and Food ScienceUniversity of NottinghamSutton Bonington, LoughboroughUK

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