New Activated PEG Derivatives for Affinity Partitioning
If affinity partitioning is to be applied industrially, it is likely that the chemistry of polymer-ligand coupling will be critical to commercial viability. Factors to be considered include reagent cost, ease of handling, loss of ligand activity, reactivity of intermediates, and linkage stability. Consequently, a great deal of effort has been invested in PEG chemistry [1,2]. This same chemistry is pertinent to other areas such as catalysis, biosensors, and coatings for control of wetting, electroosmosis, and protein adsorption [3,4]. In this article we describe recent work in three areas: (1) effects of known methods for PEG-protein coupling on protein activity and stability, (2) synthesis of heterofunctional PEG derivatives, and (3) preparation and utility of PEG aldehyde.
KeywordsCrown Ether Alkaline Phosphatase Activity Reductive Amination Monomethyl Ether Ceric Ammonium Nitrate
Unable to display preview. Download preview PDF.
- 2.J.M. Harris and M. Yalpani, Polymer-ligands used in biospecific affinity partitioning and their synthesis, in: “Partitioning in Aqueous Two Phase Systems,” H. Walter, D.E. Brooks and D. Fisher, eds., Academic Press, New York (1985)Google Scholar
- 4.J.M. Van Alstine, J.M. Harris, R.S. Snyder, P.A. Curreri, S. Bamberger and D.E. Brooks, Separation of aqueous two-phase polymer systems in microgravity, Eur. Space Agency SP-222:309 (1984)Google Scholar