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New Activated PEG Derivatives for Affinity Partitioning

  • J. M. Harris
  • K. Yoshinaga
  • M. S. Paley
  • M. R. Herati

Abstract

If affinity partitioning is to be applied industrially, it is likely that the chemistry of polymer-ligand coupling will be critical to commercial viability. Factors to be considered include reagent cost, ease of handling, loss of ligand activity, reactivity of intermediates, and linkage stability. Consequently, a great deal of effort has been invested in PEG chemistry [1,2]. This same chemistry is pertinent to other areas such as catalysis, biosensors, and coatings for control of wetting, electroosmosis, and protein adsorption [3,4]. In this article we describe recent work in three areas: (1) effects of known methods for PEG-protein coupling on protein activity and stability, (2) synthesis of heterofunctional PEG derivatives, and (3) preparation and utility of PEG aldehyde.

Keywords

Crown Ether Alkaline Phosphatase Activity Reductive Amination Monomethyl Ether Ceric Ammonium Nitrate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    J.M. Harris, Synthesis of poly (ethylene glycol) derivatives, J. Macromol. Sci.-Rev. Macromol. Chem. Phys. C25:325 (1985)CrossRefGoogle Scholar
  2. 2.
    J.M. Harris and M. Yalpani, Polymer-ligands used in biospecific affinity partitioning and their synthesis, in: “Partitioning in Aqueous Two Phase Systems,” H. Walter, D.E. Brooks and D. Fisher, eds., Academic Press, New York (1985)Google Scholar
  3. 3.
    B.J. Herren, S.G. Shafer, J.M. Van Alstine, J.M. Harris and R.S. Snyder, Control of electroosmosis in coated quartz capillaries, J. Colloid Interface Sci. 51:46 (1987)CrossRefGoogle Scholar
  4. 4.
    J.M. Van Alstine, J.M. Harris, R.S. Snyder, P.A. Curreri, S. Bamberger and D.E. Brooks, Separation of aqueous two-phase polymer systems in microgravity, Eur. Space Agency SP-222:309 (1984)Google Scholar
  5. 5.
    R.B. McComb, CN. Bowers, Jr. and S. Posen, “Alkaline Phosphatase,” Plenum, New York (1979)CrossRefGoogle Scholar
  6. 6.
    S.G. Shafer and J.M. Harris, Preparation of cyanuric-chloride activated poly(ethyleneglycol), J. Polym. Sci. Polym. Chem. Edn. 24:375 (1986)CrossRefGoogle Scholar
  7. 7.
    K. Yoshinaga, S.G. Shafer and J.M. Harris, Effects of poly(ethyleneglycol) substitution on enzyme activity, J. Bioact. Compatible Polym. 2:49 (1987)CrossRefGoogle Scholar
  8. 8.
    L.J. Karr, S.G. Shafer, J.M. Harris, J.M. Van Alstine and R.S. Snyder, Immuno-affinity partition of cells in aqueous polymer two-phase systems, J. Chromatography 354:269 (1986)CrossRefGoogle Scholar
  9. 9.
    Y.K. Park, A. Abuchowski, S. Davis and F. Davis, Pharmacology of Escherichia Coli-I-Asparaginase poly(ethyleneglycol) adduct, Anticancer Research 1:373 (1981)PubMedGoogle Scholar
  10. 10.
    C.O. Beauchamp, S.L. Gonias, D.P. Manapace and S.V. Pizzo, A new procedure for the synthesis of poly(ethyleneglycol)-protein adducts, Anal. Biochem. 131:25 (1983)PubMedCrossRefGoogle Scholar
  11. 11.
    K. Nilsson and K. Mosbach, Immobilisation of ligands with organic sulfonyl chlorides, Methods in Enzymology 104:56 (1984)PubMedCrossRefGoogle Scholar
  12. 12.
    P. Ferruti, M.C. Tanzi, L. Rusconi and R. Cecchi, Succinic half-esters of PEGs and their derivatives as oligomeric drug-binding matrices, Makromol. Chem. 182:2183 (1981)CrossRefGoogle Scholar
  13. 13.
    E. Boccu, G.P. Velo and F.M. Veronese, Pharmocokinetic properties of poly(ethyleneglycol) derivatised Superoxide dismutase, Pharmacol. Res. Commun. 14:113 (1982)PubMedCrossRefGoogle Scholar
  14. 14.
    M.D. Shalati and C.G. Overberger, Grafting of living poly(ethylene oxide) onto polystyrene via aromatic nucleophilic displacement of activated nitro groups, J. Polym. Sci. Polym. Chem. Edn. 21:3425 (1983)CrossRefGoogle Scholar
  15. 15.
    C.D. Eisenbach, M. Peuscher, G. Wegner and M. Weiss, Synthesis of poly(ethylene oxide) with unimodal and narrow molecular weight distribution, Makromol. Chem. 184:2313 (1983)CrossRefGoogle Scholar
  16. 16.
    J.M. Harris, M. Yalpani, J.M. Van Alstine, E.C. Struck, M.C. Case, M.S. Paley and D.E. Brooks, Synthesis of poly(ethyleneglycol) derivatives, J. Poly. Sci. Poly. Chem. Ed. 22:341 (1984)CrossRefGoogle Scholar
  17. 17.
    M.S. Paley and J.M. Harris, Synthesis of the aldehyde of oligomeric polyoxyethylene, J. Polym. Sci. Polvm. Chem. Edn. 25:2447 (1987)CrossRefGoogle Scholar
  18. 18.
    E. Bayer, H. Zheng and K. Geckeler, Functionalisation of soluble polymers, Polymer Bulletin 8:585 (1982)CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • J. M. Harris
    • 1
  • K. Yoshinaga
    • 1
  • M. S. Paley
    • 1
  • M. R. Herati
    • 1
  1. 1.Department of ChemistryUniversity of Alabama in HuntsvilleHuntsvilleUSA

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