New Activated PEG Derivatives for Affinity Partitioning

  • J. M. Harris
  • K. Yoshinaga
  • M. S. Paley
  • M. R. Herati


If affinity partitioning is to be applied industrially, it is likely that the chemistry of polymer-ligand coupling will be critical to commercial viability. Factors to be considered include reagent cost, ease of handling, loss of ligand activity, reactivity of intermediates, and linkage stability. Consequently, a great deal of effort has been invested in PEG chemistry [1,2]. This same chemistry is pertinent to other areas such as catalysis, biosensors, and coatings for control of wetting, electroosmosis, and protein adsorption [3,4]. In this article we describe recent work in three areas: (1) effects of known methods for PEG-protein coupling on protein activity and stability, (2) synthesis of heterofunctional PEG derivatives, and (3) preparation and utility of PEG aldehyde.


Crown Ether Alkaline Phosphatase Activity Reductive Amination Monomethyl Ether Ceric Ammonium Nitrate 
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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • J. M. Harris
    • 1
  • K. Yoshinaga
    • 1
  • M. S. Paley
    • 1
  • M. R. Herati
    • 1
  1. 1.Department of ChemistryUniversity of Alabama in HuntsvilleHuntsvilleUSA

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