Cell Separation by Immunoaffinity Partition in Aqueous Polymer Two-Phase Systems
Two methods for immunoaffinity partitioning are described. In the first, poly(ethylene glycol) (PEG) was covalently coupled to immunoglobulin G antibody preparations directed toward surface antigens unique to either sheep or human erythrocytes. The modified antibody preparations exhibited a reduction in ability to agglutinate cells and increased affinity for the PEG-rich upper phase in Dextran-PEG aqueous two-phase systems. When either preparation was incubated with a mixture of target and control erythrocytes, the partition of the erythrocyte population bearing the complimentary antigen was selectively increased into the upper phase while the control cells remained unaffected. Effective separation of cell populations was obtained in 2 hours using 30-transfer countercurrent distribution.
KeywordsCell Separation Antibody Preparation Cyanuric Chloride Cell Partition Human RBCs
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