Second Immunoaffinity Ligands for Cell Separation

  • S. Jill Stocks
  • Donald E. Brooks


Recent developments in immunoaffinity partition have included the use of a more general second affinity ligand and monoclonal antibodies. In this paper, poly(ethylene glycol) (PEG) and trypan-blue derivatised antibodies are used as second affinity ligands in two-phase systems containing PEG and dextran to immunospecifically increase the partition coefficient of human erythrocytes. In order to extend the technique towards future clinical applications, a more stringent separation problem involving transformed mouse lymphocytes is being approached. In this case a biotin-antibody ligand is employed. All the ligands were able to immunospecifically increase cell partition.


Trypan Blue Human Erythrocyte Phase System Cell Separation Separation Problem 
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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • S. Jill Stocks
    • 1
  • Donald E. Brooks
    • 1
  1. 1.Departments of Pathology and Chemistry Department of PathologyVancouverCanada

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