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Second Immunoaffinity Ligands for Cell Separation

  • S. Jill Stocks
  • Donald E. Brooks

Abstract

Recent developments in immunoaffinity partition have included the use of a more general second affinity ligand and monoclonal antibodies. In this paper, poly(ethylene glycol) (PEG) and trypan-blue derivatised antibodies are used as second affinity ligands in two-phase systems containing PEG and dextran to immunospecifically increase the partition coefficient of human erythrocytes. In order to extend the technique towards future clinical applications, a more stringent separation problem involving transformed mouse lymphocytes is being approached. In this case a biotin-antibody ligand is employed. All the ligands were able to immunospecifically increase cell partition.

Keywords

Trypan Blue Human Erythrocyte Phase System Cell Separation Separation Problem 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • S. Jill Stocks
    • 1
  • Donald E. Brooks
    • 1
  1. 1.Departments of Pathology and Chemistry Department of PathologyVancouverCanada

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