Abstract
Recent developments in immunoaffinity partition have included the use of a more general second affinity ligand and monoclonal antibodies. In this paper, poly(ethylene glycol) (PEG) and trypan-blue derivatised antibodies are used as second affinity ligands in two-phase systems containing PEG and dextran to immunospecifically increase the partition coefficient of human erythrocytes. In order to extend the technique towards future clinical applications, a more stringent separation problem involving transformed mouse lymphocytes is being approached. In this case a biotin-antibody ligand is employed. All the ligands were able to immunospecifically increase cell partition.
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© 1989 Plenum Press, New York
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Stocks, S.J., Brooks, D.E. (1989). Second Immunoaffinity Ligands for Cell Separation. In: Fisher, D., Sutherland, I.A. (eds) Separations Using Aqueous Phase Systems. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5667-7_33
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DOI: https://doi.org/10.1007/978-1-4684-5667-7_33
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5669-1
Online ISBN: 978-1-4684-5667-7
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