Second Immunoaffinity Ligands for Cell Separation
Recent developments in immunoaffinity partition have included the use of a more general second affinity ligand and monoclonal antibodies. In this paper, poly(ethylene glycol) (PEG) and trypan-blue derivatised antibodies are used as second affinity ligands in two-phase systems containing PEG and dextran to immunospecifically increase the partition coefficient of human erythrocytes. In order to extend the technique towards future clinical applications, a more stringent separation problem involving transformed mouse lymphocytes is being approached. In this case a biotin-antibody ligand is employed. All the ligands were able to immunospecifically increase cell partition.
KeywordsTrypan Blue Human Erythrocyte Phase System Cell Separation Separation Problem
Unable to display preview. Download preview PDF.
- 3.S.J. Stocks, Development of a General Ligand for Immunoaffinity Partitioning in Two Phase Aqueous Systems, Anal. Biochem., 172. in press (1988)Google Scholar
- 4.F.J. Takei, Two surface antigens expressed on proliferating mouse T lymphocytes defined by rat monoclonal antibodies, Immunol. 130:6:2794 (1983)Google Scholar
- 5.P. Chan and F.J. Takei, Expression of a T cell receptor-like molecule on normal and malignant murine T cells detected by rat monoclonal antibodies to nonclonotypic determinants, Immunol. 136:4:1346 (1986)Google Scholar
- 6.J.W. Goding, in: Monoclonal Antibodies: Principles and Practice, Academic Press, New York (1983)Google Scholar