Attempts to Fractionate Rat Bone Marrow Cells by Countercurrent Distribution (CCD) in Dextran/Polyethylene Glycol Two-Phase Systems
Cell suspension of bone marrow contains a mixture of different haematopoietic cell types. This population then provides a suitable sample to be used as a model system to study the fractionation of heterogeneous cell populations [1–3]. Bone marrow (from femur and tibia) and erythrocytes were prepared from male adult Wistar rats . Reticulocytes were from anaemic (phenylhidrazinic rats) . Dextran T-500 (Pharmacia) and poly(ethylene-glycol) PEG-6000 (Serva) were used for the preparation of two-phase systems [4,5]. Various versions of the countercurrent distribution (CCD) apparatus with 60-cavity rotors [4,5], made at the Universities of Madrid, Umeå and Sheffield, were employed. G values were obtained from equation: G=i/n-i (n=55 transfers).
KeywordsGlycol Fractionation Macromolecule Dextran Settling
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