Dye Affinity Partitioning of Serum Proteins
Affinity partitioning in aqueous two-phase systems has been applied to study the interaction of serum proteins with certain dyes. By monitoring the partition coefficient any interaction between proteins and dyes and its alteration under certain conditions can easily be recognized. In detail, the binding of Cibacron Blue F3G-A and Remazol Yellow GGL to albumin and prealbumin was studied. The influence of competing ligands and of dye modification on affinity partitioning of prealbumin was demonstrated. Furthermore, affinity partitioning in three-phase systems composed of PEG, Ficoll and dextran is shown. Attempts were made to fractionate serum proteins by countercurrent distribution using polymer-bound reactive dyes.
KeywordsPartition Coefficient Phase System Affinity Ligand Bottom Phase Middle Phase
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