Problems of White Cell Separation Techniques in Relation to the Phase Partitioning of Neutrophils

  • Paul Eggleton
  • Neville Crawford
  • Derek Fisher


The method selected for the isolation of neutrophils from peripheral blood influences the partition behaviour of the cells obtained. The phase forming polymers themselves can, to some extent, stimulate and alter the oxidative responsiveness of neutrophils. Care in the selection of batches of polymers for phase systems is clearly indicated.


Differential Centrifugation Counter Current Distribution Partition Behaviour Polyethylene Glycol Solution Neutrophil Population 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    C.E. McCall, D.A. Bass, L.R. DeChatelet, A.R. Link, and M. Ann, In vitro responses of human neutrophils to N-formyl-methionyl-leucyl-phenylalanine: correlation with effects of acutebacterial infection, J. Infect. Dis. 140:277 (1979)PubMedCrossRefGoogle Scholar
  2. 2.
    J.E. Repine, C.C. Clawson, and F.C. Goetz, Bactericidal function of neutrophils from patients with acute bacterial infections and from diabetics, J. Infect. Dis. 142:869 (1980)PubMedCrossRefGoogle Scholar
  3. 3.
    F.R Venezio, G.O. Westenfelder, and J.P. Phair, The adherence of polymorphonuclear leukocytes in patients with sepsis, J. Infect. Dis. 145:351 (1982)PubMedCrossRefGoogle Scholar
  4. 4.
    R.L. Baehner, and L.A. Boxer, Pisorders of polymorphonuclear leukocyte function related to alterations in the integrated reactions of cytoplasmoic constituents with the plasma membrane. Seminars in Hematol. 16:148 (1979)Google Scholar
  5. 5.
    F.S. Southwick, and T.P. Stossel, Contractile proteins in leukocyte function. Seminars in Hematol. 20:305 (1983)Google Scholar
  6. 6.
    P. Eggleton, I.A. Sutherland and P. Fisher, Rapid partition of human neutrophils by CCP, in: “Separations Using Aqueous Phase Systems: Applications in Cell Biology and Biotechnology,” P. Fisher and I.A. Sutherland, eds., Plenum, New York (1989)Google Scholar
  7. 7.
    C. Jarstrand, G. Lahnborg and L. Berhem, Human granulocyte function during dextran infusion, Acta Chir. Scand. 489:279 (1979)Google Scholar
  8. 8.
    R.D. Nelson, P.G. Quie and R.L. Simmons, Chemotaxis under agarose: A new and simple method for measuring chemotaxis and spontaneous migration of human polymorphonuclear leukocytes and monocytes, J. Immunol. 115:1650 (1975)PubMedGoogle Scholar
  9. 9.
    L. Poston, R.B. Jones and P.J. Hilton, Sodium transport of polymorphonuclear leukocytes; effects of isolation by the Ficoll/Triosil method, Clin. Sci. 62:563 (1982)PubMedGoogle Scholar
  10. 10.
    J.A. Roth and M.L. Kaeberle, Isolation of neutrophils and eosinophils from the peripheral blood of cattle and comparison of their functional activities, J. Immunol Methods. 45:153 (1981)PubMedCrossRefGoogle Scholar
  11. 11.
    N. Dioguardi, A. Agostini, G. Fiorelli and B. Lomanto, Characterisation of lactic dehydrogenase of normal human granulocytes. J. Lab. Clin. Med. 61: 713 (1963).PubMedGoogle Scholar
  12. 12.
    P. Eggleton, Rapid techniques for the measurement of non-specific host responses to bacterial infection, M.Phil. Thesis, University of London (1987)Google Scholar
  13. 13.
    B. Babior, R.S. Kipnes and J.T. Curnutte, The production by leukocytes of Superoxide, a potential bacterial agent, J. Clin. Invest. 52:741 (1973)PubMedCrossRefGoogle Scholar
  14. 14.
    H. Walter, D.E. Brooks, and D. Fisher, “Partitioning in Aqueous Two-Phase Systems. Theory, Methods, Uses and Applications to Biotechnology,” Academic Press Inc., (1985).Google Scholar
  15. 15.
    R.S. Hill, R. Norris-Jones, B. Still and D.E. Brooks, Surface charge and hydrophobic properties of fresh and cyropreserved blood phagocytes as determined by partitioning in two-phase aqueous polymer systems, Am. J. Hematol. 21:249 (1986)PubMedCrossRefGoogle Scholar
  16. 16.
    D. Fisher, Mechanisms of cell fusion induced by polyethylene glycol, in: “Microorganisms in Aqueous Two-Phase Systems,” IVA-rapport 330, Royal Swedish Academy of Engineering Sciences, Stockholm (1986)Google Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Paul Eggleton
    • 1
  • Neville Crawford
    • 2
  • Derek Fisher
    • 2
  1. 1.Biochemistry Department, Royal Free Hospital School of MedicineUniversity of LondonLondonUK
  2. 2.Biochemistry DepartmentRoyal College of Surgeons of EnglandLondonUK

Personalised recommendations