Problems of White Cell Separation Techniques in Relation to the Phase Partitioning of Neutrophils
The method selected for the isolation of neutrophils from peripheral blood influences the partition behaviour of the cells obtained. The phase forming polymers themselves can, to some extent, stimulate and alter the oxidative responsiveness of neutrophils. Care in the selection of batches of polymers for phase systems is clearly indicated.
KeywordsDifferential Centrifugation Counter Current Distribution Partition Behaviour Polyethylene Glycol Solution Neutrophil Population
Unable to display preview. Download preview PDF.
- 4.R.L. Baehner, and L.A. Boxer, Pisorders of polymorphonuclear leukocyte function related to alterations in the integrated reactions of cytoplasmoic constituents with the plasma membrane. Seminars in Hematol. 16:148 (1979)Google Scholar
- 5.F.S. Southwick, and T.P. Stossel, Contractile proteins in leukocyte function. Seminars in Hematol. 20:305 (1983)Google Scholar
- 6.P. Eggleton, I.A. Sutherland and P. Fisher, Rapid partition of human neutrophils by CCP, in: “Separations Using Aqueous Phase Systems: Applications in Cell Biology and Biotechnology,” P. Fisher and I.A. Sutherland, eds., Plenum, New York (1989)Google Scholar
- 7.C. Jarstrand, G. Lahnborg and L. Berhem, Human granulocyte function during dextran infusion, Acta Chir. Scand. 489:279 (1979)Google Scholar
- 12.P. Eggleton, Rapid techniques for the measurement of non-specific host responses to bacterial infection, M.Phil. Thesis, University of London (1987)Google Scholar
- 14.H. Walter, D.E. Brooks, and D. Fisher, “Partitioning in Aqueous Two-Phase Systems. Theory, Methods, Uses and Applications to Biotechnology,” Academic Press Inc., (1985).Google Scholar
- 16.D. Fisher, Mechanisms of cell fusion induced by polyethylene glycol, in: “Microorganisms in Aqueous Two-Phase Systems,” IVA-rapport 330, Royal Swedish Academy of Engineering Sciences, Stockholm (1986)Google Scholar