Separation of Chondrocytes in Aqueous Two-Phase Systems
Chondrocytes were isolated from bovine articular cartilage and put into a suspension culture. By using CCD the partition behaviour of the chondrocytes as followed at various times in culture. Freshly isolated cells are partitioned into one peak consisting of single cells. During culture two new peaks appeared in the CCD both of which contained cell aggregates of different kinds. By modifying the chemical composition of the cell surface it is demonstrated that the partition is regulated by the presence of proteoglycans at the cell surface. Besides affecting the charge density of the cells, increased concentration of proteoglycans also produces an increased cell aggregation.
KeywordsArticular Cartilage Hyaluronic Acid Suspension Culture Cartilage Proteoglycan Partition Behaviour
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