Separation and Subfractionation of Cell Populations by Phase Partitioning — An Overview

  • Derek Fisher

Abstract

The general principles of cell partitioning in aqueous two-phase systems are reviewed. Particular attention is given to the ability of countercurrent distribution (CCD) to subfractionate cell populations which by other means may seem to be homogeneous. General strategies for demonstrating heterogeneity in cell populations are outlined and examples provided from recent studies on K-562 cells by Walter and his colleagues. The influence of the conditions of phase separation upon the efficiency of cell fractionation is discussed.

Keywords

Electrophoresis Fractionation Radioactive Isotope Beach Macromolecule 

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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Derek Fisher
    • 1
  1. 1.Department of Biochemistry and Chemistry, Royal Free Hospital School of MedicineUniversity of LondonLondonUK

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