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Isolation of Plasma Membrane Vesicles from Fat Cells of Epididymal Fat Pads of the Rat by Aqueous Two-Phase Partition

  • Dorothy M. Morré
  • D. James Morré
  • Timothy Snyder
  • William Li
  • Amy Erb
  • David W. Sammons

Abstract

The responses of fat cells to insulin involve potentially important membrane alterations. There is now considerable evidence to support the concept that insulin stimulates glucose transport by inducing a rapid and reversible translocation of glucose transporters from an intracellular membrane pool to the plasma membrane [1–4]. However, little is known about the cellular mechanism of insulin and the process or biochemical events whereby glucose transporter levels at the cell surface may be modulated. Initial efforts [5] to better characterise the glucose transport system involved use of a sensitive silver staining method to visualise membrane proteins in two-dimensional gel electrophoretograms in conjunction with computerised scanning and quantification. A 90 kDa protein was lost from the plasma membranes upon incubation of fat cells with insulin and calcium. We are investigating the dynamics and identity of the 90 kDa protein in the fat cell, and especially the mechanism whereby the protein is dissociated from the plasma membrane as well as its fate and function in relation to the oncoming glucose transporter vesicles from the cytoplasm.

Keywords

Adenosine Triphosphatase Plasma Membrane Vesicle Insulin Stimulate Glucose Transport Glucose Transport System Purify Plasma Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Dorothy M. Morré
    • 1
    • 2
  • D. James Morré
    • 1
    • 2
  • Timothy Snyder
    • 1
    • 2
  • William Li
    • 1
    • 2
  • Amy Erb
    • 1
    • 2
  • David W. Sammons
    • 1
    • 2
  1. 1.Department of Foods and Nutrition, Department of Medicinal ChemistryPurdue UniversityW. LafayetteUSA
  2. 2.Center for Separation ScienceThe University of ArizonaTucsonUSA

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