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Affinity Partitioning and Centrifugal Countercurrent Distribution of Synaptosomes from Calf Brain Cortex

  • B. Olde
  • G. Johansson

Abstract

The aim of this work was to test the possibility of using affinity partitioning, in combination with centrifugal-CCD, for fractionation of a crude synaptosomal preparation.

Keywords

Glutamine Synthetase Phase System High Specific Activity Membrane Domain Cholinergic Receptor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    G. Johansson, R. Gysin and S.D. Flanagan, Affinity partitioning of membranes. Evidence for discrete membrane domains containing cholinergic receptors, J. Biol. Chem. 256:9126 (1981)PubMedGoogle Scholar
  2. 2.
    K. Haga and T. Haga, Affinity chromatography of the muscarine acetylcholine receptor, J. Biol. Chem. 258:13575 (1983)PubMedGoogle Scholar
  3. 3.
    M.J. Lopez-Perez, G. Paris and C. Larsson, Highly purified mitochondria from rat brain prepared by phase partition, Biochem. Biophys. Acta 635:359 (1981)PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • B. Olde
    • 1
  • G. Johansson
    • 1
  1. 1.Department of BiochemistryChemical CenterLundSweden

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