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Plasma Membranes from Hepatomas and Cultured Cells by Aqueous Two-Phase Partition

  • D. David Nowack
  • Dorothy M. Morré
  • D. James Morré

Abstract

Neoplastic cultured cells and tissues often exhibit altered plasma membrane morphologies and biochemical activities when compared to non-transformed counterparts [1]. However, accurate interpretation and reliability of data collected for such comparisons may be influenced greatly by the purity and composition of the plasma membrane fractions utilised. Non-uniform homogenisation, contamination by organelles other than plasma membrane and changes in culture conditions, together with the transformation process itself, all tend to confound the fractionation of plasma membranes from cells and hepatomas.

Keywords

Enzymatic Marker Adenosine Triphosphatase Plasma Membrane Fraction Plasma Membrane Vesicle Swing Bucket Rotor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    D. Wallach, Membrane Molecular Biology of Neoplastic Cells, Elsevier, New York (1975)Google Scholar
  2. 2.
    S. Kashiwampta, S. Goto, R. Samba and F. Suzuki, Inhibition by bilirubin of (Na+-K+)-activated adenosine triphosphatase and K+-activated p-nitrophenyl phosphatase activities of nal-treated microsomes from young rat cerebrum J. Biol. Chem. 254:4577 (1979)Google Scholar
  3. 3.
    E.M. Croze and D.J. Morré, Isolation of plasma membrane, Golgi apparatus, and endoplasmic reticulum fractions from single homogenates of mouse liver, J. Cell Physiol 119:46 (1984)PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • D. David Nowack
    • 1
  • Dorothy M. Morré
    • 1
  • D. James Morré
    • 1
  1. 1.Department of Foods and Nutrition and Department of Medicinal Chemistry and PharmacognosyPurdue UniversityWest LafayetteUSA

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