Plasma Membranes from Hepatomas and Cultured Cells by Aqueous Two-Phase Partition
Neoplastic cultured cells and tissues often exhibit altered plasma membrane morphologies and biochemical activities when compared to non-transformed counterparts . However, accurate interpretation and reliability of data collected for such comparisons may be influenced greatly by the purity and composition of the plasma membrane fractions utilised. Non-uniform homogenisation, contamination by organelles other than plasma membrane and changes in culture conditions, together with the transformation process itself, all tend to confound the fractionation of plasma membranes from cells and hepatomas.
KeywordsEnzymatic Marker Adenosine Triphosphatase Plasma Membrane Fraction Plasma Membrane Vesicle Swing Bucket Rotor
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