Two-Phase Partitioning of Rat Liver Plasma Membranes
Rat liver plasma membranes were subjected to countercurrent distribution in a dextran/poly(ethylene glycol) two-phase system. The lateral heterogeneity was examined by plotting the specific activities of marker components, for each distribution fraction, against each other. The patterns obtained were compared with those of different model membranes. By this method 5′-nucleotidase and leucine amino-peptidase were found in separate but adjacent domains, whereas asialo orosomucoid receptors resided in a domain separated from those containing 5′-nucleotidase or leucine aminopeptidase by a domain lacking these markers.
KeywordsFragment Size Leucine Aminopeptidase Lateral Heterogeneity Countercurrent Distribution Hepatocyte Plasma Membrane
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