Use of Phase Partitioning in Multidimensional Subcellular Fractionation
Conventional subcellular fractionation procedures incorporate two separation dimensions, one based on membrane sedimentation coefficient, the other on equilibrium density. Questions about the homogeneity of samples obtained with these procedures have been addressed with additional separations based on manipulation of membrane density or on partitioning in dextran-poly(ethylene glycol) two-phase systems. In some cases it has proven useful to employ a total of 4 separation dimensions, i.e. sedimentation, equilibrium density in 2 differently designed density gradients, and phase partitioning. In other cases, parallel third dimensions analyses in two-phase systems with different pH values have been used to delineate the multiplicity of populations present in conventional subcellular samples.
KeywordsAcid Phosphatase Apical Membrane Lacrimal Gland Separation Dimension Phase Partitioning
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