Abstract
Conventional subcellular fractionation procedures incorporate two separation dimensions, one based on membrane sedimentation coefficient, the other on equilibrium density. Questions about the homogeneity of samples obtained with these procedures have been addressed with additional separations based on manipulation of membrane density or on partitioning in dextran-poly(ethylene glycol) two-phase systems. In some cases it has proven useful to employ a total of 4 separation dimensions, i.e. sedimentation, equilibrium density in 2 differently designed density gradients, and phase partitioning. In other cases, parallel third dimensions analyses in two-phase systems with different pH values have been used to delineate the multiplicity of populations present in conventional subcellular samples.
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References
C. DeDuve, Principles of tissue fractionation, J. Theor. Biol. 6:33 (1964)
N.G. Anderson, W.W. Harris, A.A. Barber, C.T. Rankin and E.L. Chandler, “Separation of Subcellular Components by Combined Rate and Isopycnic Zonal Centrifugation,” National Cancer Institute Monograph 26, U.S. Government Printing Office, Washington, D.C. (1966)
G. Saccomani, H.B. Stewart, D. Shaw, M. Lewin and G. Sachs, Characterisation of gastric mucosal membranes. IX. Fractionation by zonal centrifugation and free flow electrophoresis technique, Biochim. Biophys. Acta 456:311 (1977)
A.K. Mircheff, Empirical strategy for analytical fractionation of epithelial cells, Am. J. Physiol. 244:G347 (1983)
A.K. Mircheff and C.C. Lu, A map of membrane populations isolated from rat exorbital gland, Am. J. Physiol. 247:G651 (1984)
A.K. Mircheff, D.J. Ahnen, A. Islam, N.A. Santiago and G.M. Gray, Complex subcellular distributions of enzymatic markers in intestinal epithelial cells, J. Membrane Biol. 83:95 (1985)
D.J. Ahnen, A.K. Mircheff, N.A. Santiago, C. Yoshioka and G.M. Gray, Intestinal surface aminooligopeptidase. Distinct molecular forms during assembly on intracellular membranes in vivo, J. Biol. Chem. 258:5960 (1983)
C.N. Conteas, A.A. McDonough, T.R. Kozlowski, C.B. Hensley, R.L. Wood and A.K. Mircheff, Mapping subcellular distribution of Na+-K+-ATPase in rat parotid gland, Am. J. Physiol. 250:C430 (1986)
R.W. Lambert, M.E. Bradley and A.K. Mircheff, Sodium/proton and chloride/bicarbonate antiporters in rat lacrimal gland basal-lateral membranes, Invest. Ophthalmol. Vis. Sci. 28s:156 (1987)
C.L. Peters, M.E. Bradley, S.C. Yiu and A.K. Mircheff, Muscarinic receptors in cell surface and intracellular membranes of rat exorbital lacrimal gland, Invest. Ophthalmol. Vis. Sci. 27s:26 (1986)
S. Grinstein and A. Rothstein, Mechanisms of regulation of the Na+/H+ exchanger, J. Membrane Biol. 90:1 (1986)
H.E. Ives, V.J. Yee and D.J. Warnock, Asymmetric distribution of the Na+/H+ in renal proximal tubule epithelial cells, J. Biol. Chem. 258:13513 (1983)
A.K. Mircheff, H.E. Ives, V.J. Yee and D.G. Warnock, Na+/H+ antiporter in membrane populations resolved from a renal brush border vesicle preparation, Am. J. Physiol. 246:F843 (1984)
E.J.J.M. van Corven, C.H. van Os and A.K. Mircheff, Subcellular distribution of ATP-dependent calcium transport in rat duodenal epithelium, Biochim. Biophys. Acta 861:267 (1986)
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© 1989 Plenum Press, New York
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Mircheff, A.K., Bradley, M.E., Hensley, C.B., van Corven, E.J.J.M., Yiu, S.C., Lambert, R.W. (1989). Use of Phase Partitioning in Multidimensional Subcellular Fractionation. In: Fisher, D., Sutherland, I.A. (eds) Separations Using Aqueous Phase Systems. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5667-7_14
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DOI: https://doi.org/10.1007/978-1-4684-5667-7_14
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