Raising Monoclonal Antibodies Against Plasma Membranes from Barley Embryos
In vitro propagation and genetic manipulation of plants have vast potentialities for modern breeding. Cultured cells, transgenic or of natural genotype, may be induced to form somatic embryos, leading to a final regeneration into plants with the desired characters. However, regeneration competence is not inherent in every cell, and with the fact that several important crop species are still recalcitrant this calls for studies of embryogenesis and cell differentiation. Barley (Hordeum vulgare L.) presents severe obstacles to regeneration, as do most of the cereals. Besides trying out different regeneration techniques for this species , molecular markers specific to embryongenic cells are searched for. Some of the markers are likely to be present on the cell surface, and hence the plasma membrane (PM) is to be studied, in our case by means of immunological methods using monoclonal antibodies.
KeywordsSomatic Embryo Golden Promise Phase Partitioning Severe Obstacle Natural Genotype
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