Raising Monoclonal Antibodies Against Plasma Membranes from Barley Embryos

  • Ole Schou

Abstract

In vitro propagation and genetic manipulation of plants have vast potentialities for modern breeding. Cultured cells, transgenic or of natural genotype, may be induced to form somatic embryos, leading to a final regeneration into plants with the desired characters. However, regeneration competence is not inherent in every cell, and with the fact that several important crop species are still recalcitrant this calls for studies of embryogenesis and cell differentiation. Barley (Hordeum vulgare L.) presents severe obstacles to regeneration, as do most of the cereals. Besides trying out different regeneration techniques for this species [1], molecular markers specific to embryongenic cells are searched for. Some of the markers are likely to be present on the cell surface, and hence the plasma membrane (PM) is to be studied, in our case by means of immunological methods using monoclonal antibodies.

Keywords

Chlorophyll Ethylene Glycol Polypeptide Dextran 

References

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    P. Kjellbom and C. Larsson, Physiol. Plant 62:501 (1984)CrossRefGoogle Scholar
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    J.L. Hall and A.L. Moore (eds) Isolation of membranes and organelles from plant cells, Academic Press, London (1983)Google Scholar
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    J.W. Goding, Monoclonal antibodies: Principles and practice Academic Press, London (1983)Google Scholar
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    H.D. Grimes et al, Biochim Biophvs Acta 862:165 (1986)CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Ole Schou
    • 1
  1. 1.Agricultural Research DepartmentRiso National LaboratoryRoskildeDenmark

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