Plasma Membrane Isolation by Phase Partition using a Single Partition Step: A Tool for the in vivo Study of Lipid Transfer to the Plasma Membrane
The intermembrane transfer of very long chain fatty acids (VLCFA) from a light membrane fraction (1.08–1.09 g/cm3) to heavier membranes (1.16–1.18 g/cm3) was demonstrated in etiolated leek seedlings . By means of biochemical markers, it was determined that the plasma membrane is confined to the 1.16–1.18 g/cm3 fraction which is, nevertheless, heterogeneous. The plasma membrane was further purified in an aqueous two-polymer phase system, its characterisation is presented. Following pulse-chase experiments, an increase of labeled fatty acids (C16 to C24) in the plasma membrane occurred during the chase, demonstrating the transfer of these molecules to the plasma membrane.
KeywordsLower Phase Plasma Membrane Fraction Plasma Membrane Vesicle Phase Partition Very Long Chain Fatty Acid
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- 3.R. Lessire, T. Abdul-Karim and C. Cassagne, Origin of the wax very long chain fatty acids in leek, Allium porrum L., leaves: a plausable model, in: “The Plant Cuticle”, D.F. Cuttler, K.L. Alvin and C.E. Price, eds., Academic Press, London, 167 (1982)Google Scholar
- 6.P. Moreau, Thèse de Doctorat de l’Université de Bordeaux II (1986)Google Scholar
- 8.A.M. Heape, H. Juguelin, M. Fabre, F. Boiron, B. Garbay, M. Fournier, J. Bonnet and C. Cassagne, Correlation between the morphology and the lipid and protein composition in the peripheral nervous system of individual 8-day old normal and trembler mice (BRD 50344), Dev. Brain Res. 25:173 (1986)CrossRefGoogle Scholar
- 16.R. Lessire, Thèse de Doctorat es Sciences, Bordeaux (1981)Google Scholar
- 17.P. Mazliak, Glyco-and phospholipids of biomembranes in higher plants, in: “Lipids and Lipids Polymers in Higher Plants”, M. Tevini and H.K. Lichtenthaler, eds., Springer-Verlag, Berlin (1977)Google Scholar
- 18.J.L. Harwood, Plant acyl lipids structure distribution and analysis, in: “The Biochemistry of Plants” (1980) P.K. Stumpf and E.E. Conn, eds., Academic Press, LondonGoogle Scholar
- 20.P. Moreau, H. Juguelin, R. Lessire and C. Cassagne, Study of the intracellular transfer of lipids to the plasmalemma, in: “The Metabolism, Structure and Function of Plant Lipids”, P.K. Strumpf, J.B. Mudd and W.D. Nes, eds., Plenum Press, New-York (1987)Google Scholar
- 24.J.D. Clement, J.P. Blein, J. Rigaud and R. Scalla, Characterisation of ATPase from maize shoot plasma membrane prepared by partition in an aqueous polymer two phase system, Physiol. Veg. 24:25 (1986)Google Scholar