Plasma Membrane Isolation by Phase Partition using a Single Partition Step: A Tool for the in vivo Study of Lipid Transfer to the Plasma Membrane

  • Patrick Moreau
  • Hélène Juguelin
  • René Lessire
  • Claude Cassagne


The intermembrane transfer of very long chain fatty acids (VLCFA) from a light membrane fraction (1.08–1.09 g/cm3) to heavier membranes (1.16–1.18 g/cm3) was demonstrated in etiolated leek seedlings [1]. By means of biochemical markers, it was determined that the plasma membrane is confined to the 1.16–1.18 g/cm3 fraction which is, nevertheless, heterogeneous. The plasma membrane was further purified in an aqueous two-polymer phase system, its characterisation is presented. Following pulse-chase experiments, an increase of labeled fatty acids (C16 to C24) in the plasma membrane occurred during the chase, demonstrating the transfer of these molecules to the plasma membrane.


Lower Phase Plasma Membrane Fraction Plasma Membrane Vesicle Phase Partition Very Long Chain Fatty Acid 
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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Patrick Moreau
    • 1
  • Hélène Juguelin
    • 1
  • René Lessire
    • 1
  • Claude Cassagne
    • 1
  1. 1.Institut de Biologie Cellulaire et de NeurochimieCNRSBordeaux CedexFrance

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