tRNA Genes - Tinkering in Organization and Expression?

  • Horst Feldmann
Part of the NATO ASI Series book series (NATO ASI, volume 169)


tRNA genes and components of their basic transcription machinery provide well-documented examples for the conservation of cis-acting elements and trans-acting factors from lower to higher eukaryotes. Like other genes that are transcribed by RNA polymerase III, tRNA genes contain intragenic control regions that direct their transcription. For tRNA genes, these highly conserved internal control regions (the A and B boxes) coincide with sequences of the D-loop and the T-loop of the tRNAs (review, Sharp et al., 1985).


Transposable Element tRNA Gene Chromatin Organization Template Acti Basic Transcription Machinery 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Almer, A. and Hörz, W., 1986, Nuclease hypersensitive regions with adjacent positioned nucleosomes mark the gene boundaries of the PHO5/PHO3 locus in yeast, EMBO J. 5:2681–2687.PubMedGoogle Scholar
  2. Baker, R.E., Camier, S., Sentenac, A. and Hall, B.D., 1987, Gene size differentially affects the binding of yeast transcription factor tau to two intragenic regions, Proc. Natl. Acad. Sci. USA 84:8768–8772.PubMedCrossRefGoogle Scholar
  3. Baldi, M.I., Matoccia, E., Ciafrè, S., Attardi, D.G. and Tocchini-Valentini, G.P., 1986, Binding and cleavage of pre-tRNA by the Xenopus splicing endonuclease: two separable steps of the intron excision reaction, Cell 47:965–971.PubMedCrossRefGoogle Scholar
  4. DeLotto, R. and Schedl, P., 1984, Internal promoter elements of transfer RNA genes are preferentially exposed in chromatin, J. Mol. Biol. 179:607–628.PubMedCrossRefGoogle Scholar
  5. Eigel, A. and Feldmann, H., 1982, Tyl and delta elements occur adjacent to several tRNA genes in yeast, EMBO J. 1:1245–1255.PubMedGoogle Scholar
  6. Genbauffe, F.S., Chisholm, G.E. and Cooper, T.G., 1984, Tau, sigma, and delta, a family of repeated elements in yeast, J. Biol. Chem. 259:10518–10525.PubMedGoogle Scholar
  7. Greer, C.L., Soll, D. and Willis, I., 1987, Substrate recognition and identification of splice sites by the tRNA-splicing endonuclease and ligase from Saccharomyces cerevisiae, Mol. Cell. Biol. 7:76–84.PubMedGoogle Scholar
  8. Hauber, J. Nelböck, P. and Feldmann, H., 1985, Nucleotide sequence and characteristics of a Ty element from yeast, Nucleic Acids Res. 13:2745–2758.PubMedCrossRefGoogle Scholar
  9. Huibregtse, J.M., Evans, CF. and Engelke, D.R., 1987, Comparison of tRNA gene transcription complexes formed in vitro and in nuclei, Mol. Cell. Biol. 7:3212–3220.PubMedGoogle Scholar
  10. Nedopasov, S. and Georgiev, G., 1980, Non-random cleavage of SV40 DNA in the compact minichromosome and free in solution by micrococcal nuclease, Biophys. Biochem. Res. Comm. 92:532–539.CrossRefGoogle Scholar
  11. Nelböck, P., Stucka, R. and Feldmann, H., 1985, Different patterns of transposable elements in the vicinity of tRNA genes in yeast: a possible clue to transcriptional modulation, Biol. Chem. Hoppe-Seyler 366:1041–1051.PubMedCrossRefGoogle Scholar
  12. Raymond, G.J. and Johnson, J.D., 1987, The 5′flanking sequence of yeast tRNA (Leu3) genes enhances the rate of transcription from stable pre-initiation complexes, Nucleic Acids Res. 15:9881–9894.PubMedCrossRefGoogle Scholar
  13. Ruet, A., Camier, S., Smagowicz, W. Sentenac, A. and Fromageot, P., 1984, Isolation of a class C transcription factor which forms a stable complex with tRNA genes, EMBO J. 3:343–350.PubMedGoogle Scholar
  14. Sandmeyer, S.B. and Olson, M.V., 1982, Insertion of a repetititve element at the same position in the 5′—flanking regions of two dissimilar yeast tRNA genes, Proc. Natl. Acad. Sci. USA 79:7674–7678.PubMedCrossRefGoogle Scholar
  15. Sandmeyer, S.B., Bilanchone, V.W., Clark, D.J. Morcos, P. Carle, G.F. and Brodeur, G.M., 1988, Sigma elements are position-specific for many different yeast tRNA genes, Nucleic Acids Res. 16:1499–1515.PubMedCrossRefGoogle Scholar
  16. Sharp, S.J., Schaack, J., Cooley, L. Burke, D.J. and Soll, D., 1985, Structure and transcription of eukaryotic tRNA genes, CRC Critical Rev. Biochem. 19:107–144.CrossRefGoogle Scholar
  17. Wu, C., 1980, The 5′ends of Drosophila heat shock genes in chromatin are hypersensitive to DNaseI, Nature 286:854–860.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Horst Feldmann
    • 1
  1. 1.Institut für Physiologische ChemieUniversität MünchenMünchen 2Germany

Personalised recommendations