Translational Control by Phosphorylation of Mammalian Initiation Factors
Translational control is defined as a change in the efficiency of translation of mRNAs. This may involve a quantitative change in the overall amount of protein synthesized, called global control. Alternatively, the change may affect the synthesis of specific proteins, called mRNA-specific control. In either case, efficiency of translation is usually measured by radiolabeled amino acid incorporation into protein, or by the number and activity of ribosomes. Since the rate limiting step in protein synthesis appears to be at the initiation phase, most control mechanisms are thought to regulate this step. We therefore focus on the pathway of initiation and address studies of how global rates of protein synthesis may be controlled.
KeywordsInitiation Factor Translational Control eIF2a Phosphorylation Amino Acid Incorporation eIF2a Kinase
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