Yeast pre-mRNA Splicing Mutants
The removal of introns from the primary transcript occurs by RNA splicing. Three major types of RNAs; tRNA, rRNA and mRNA, are known to contain introns. There are two general questions concerning any splicing mechanism: first, how is the precise recognition and alignment of the splice junctions achieved in introns whose lengths vary from a few hundred nucleotides to several thousands of nucleotides, and second, what are the biochemical mechanisms of the cleavage and ligation reactions.
KeywordsSplice Junction Splice Machinery Splice Reaction Splice System Spliceosome Assembly
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- Pearson, N. J., Thornburn, D. C., and Haber, J. E., 1982, A supressor of temperature sensitive RNA mutations that affect general and specific messenger RNA processing in yeast, Mol. Cell Biol., w:571–577.Google Scholar
- Slotyk, A., Tropak, M., and Friesen, J. D., 1984, Isolation and characterization of the RNA2, RNA4 and RNA11 genes of Saccharomyces cerevisiae, J. Bact., 160:1093–1100.Google Scholar