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Interrelationship Among Morphology, Metabolism, and Proliferation of Tumor Cells in Monolayer and Spheroid Culture

  • Stefan Walenta
  • Anneli Bredel
  • Ulrich Karbach
  • Leoni Kunz
  • Lutz Vollratt
  • Wolfgang Mueller-Klieser
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 248)

Abstract

Previous investigations have indicated a positive correlation between the proliferative and metabolic activities of tumor cells in monolayer culture (Freyer et al., 1984; Freyer and Sutherland, 1985; Walenta and Mueller-Klieser, 1987). On the other hand, no difference in the local oxygen consumption has been found between highly proliferating outer cell areas and non-proliferating inner cellular regions in multicellular tumor spheroids (Mueller-Klieser, 1984, 1987). Therefore, the interrelationship among metabolism, proliferation, and cellular morphology was investigated systematically in tumor cells both in monolayer and spheroid culture.

Keywords

Label Index Oxygen Consumption Rate Cellular Volume Multicellular Spheroid Spheroid Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Stefan Walenta
    • 1
  • Anneli Bredel
    • 1
  • Ulrich Karbach
    • 1
  • Leoni Kunz
    • 1
  • Lutz Vollratt
    • 2
  • Wolfgang Mueller-Klieser
    • 1
  1. 1.Department of Applied PhysiologyUniversity of MainzMainzGermany
  2. 2.Department of AnatomyUniversity of MainzMainzGermany

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