31P Nuclear Magnetic Resonance Measurements of ATPase Kinetics in Malignant Tumors
The estimation of tumor growth rate is generally made on the basis of tumor histology, its site of origin, and sequential radiographic or caliper measurements of its dimensions. There are currently no techniques capable of measuring the expected growth rate non-invasively, serially and prospectively. An assay, able to measure the growth rate of a partially or fully treated tumor would be of obvious potential advantage for optimizing therapy. We propose the use of 31P nuclear magnetic resonance (NMR) magnetization transfer techniques for non-invasive and continuous estimation of tumor growth kinetics. Specifically, the rate of nucleoside triphosphate (NTP) synthesis can be quantitatively measured in vivo using 31P-NMR (1–4), and thus it is possible to accurately measure the metabolic rate of a tumor. Since higher energy consumption rates in tumors are likely to correlate with more rapid proliferation and growth (5–9), 31P magnetization transfer NMR should give an indirect indication of tumor growth rate. The purpose of this study was to examine the potential for 31P-NMR magnetization transfer experiments, measuring the kinetics of the reactions catalyzed by ATPase and creatine kinase to indirectly detect the decrease in growth rate which occurs in tumors of larger size.
KeywordsNuclear Magnetic Resonance Nuclear Magnetic Resonance Spectroscopy Magnetization Transfer Tumor Growth Rate Interpulse Interval
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