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Near Infrared Quadruple Wavelength Spectrophotometry of the Rat Head

  • Osamu Hazeki
  • Mamoru Tamura
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 248)

Abstract

Cytochrome oxidase is the terminal enzyme in the respiratory chain, which ultimately transfers electrons to molecular oxygen. Its redox state can be used as a direct indicator of the sufficiency of oxygen supply to the site of cellular energy production, the mitochondria. The enzyme when oxidized has a broad absorption band in near infrared region and this absorption disappears when its copper component is reduced. Because the extinction coefficients for most biological materials are low in near infrared region, it is not difficult to detect near infrared light that penetrated intact tissues. Thus near infrared spectroscopy is expected to be a new tool for noninvasively monitoring tissue hypoxic injury. The technique was first introduced by Jöbsis(1977), and has been applied in several clinical and physiological situations (e.g. Wiernsperger et al., 1981; Cairns et al., 1985; Ferrari et al., 1985; Wyatt et al., 1986). The main obstacle to quantitation of the cytochrome oxidase redox state in intact organ has been the overlap of the spectral changes due to variation in the amount and oxygenation state of hemoglobin. Algorithms previously reported include dual and triple wavelength analysis of difference spectrum. Wray et al. (1988) assumed the additive absorption of three major components, namely oxygenated and deoxygenated hemoglobin and the redox state of cytochrome oxidase, and calculated the change of each component by solving simultaneous equations at three separate wavelengths. The absorptivity constant of the cytochrome change was determined in fluorocarbon exchange transfused rat brain, while those of hemoglobin were measured in a clear solution. Hazeki et al. (1987) proposed a similar method where the standard spectra of hemoglobin were obtained directly in perfused rat head preparations.

Keywords

Redox State Cytochrome Oxidase Near Infrared Spectroscopy Reference Wavelength Redox Change 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Cairns, C.B., Fillipo, D. and Proctor, J. (1985) A noninvasive method for monitoring the effect of increased intracranial pressure with near infrared spectrophotometry. Surg. Gynecol. Obstet., 161: 145–148.PubMedGoogle Scholar
  2. Ferrari, M., Giannini, I., Siderei, G. and Zanette, E. (1985) Continuous non invasive monitoring of the human brain by near infrared spectroscopy. Adv. Exp. Med. Biol., 191: 873–882PubMedGoogle Scholar
  3. Hazeki, O., Sciyama, A. and Tamura, M. (1987) Near-infrared spectro-photometric monitoring of haemoglobin and cytochrome a,a3 in situ. Adv. Exp. Med. Biol., 215: 283–289PubMedGoogle Scholar
  4. Hazeki, O. and Tamura, M. (1988) Quantitative analysis of hemoglobin oxygenation state of rat brain in situ by near-infrared spectrophotometry. J. Appl. Physiol., 64: 796–802PubMedGoogle Scholar
  5. Jobsis, F.F. (1977) Noninvasive, infrared monitoring of cerebral and myocardial oxygen sufficiency and circulatory parameters. Science, 198: 1264–1267PubMedCrossRefGoogle Scholar
  6. Kariman, K. and Burkhart, D.S. (1985) Non-invasive in vivo spectro-photometric monitoring of brain cytochrome a,a3 revisited. Brain Res., 360: 203–213PubMedCrossRefGoogle Scholar
  7. Steinke, J.M. and Shepherd, A.P. (1986) Role of light scattering in whole blood oximetry. IEEE Trans. Biomed. Eng., BME-33: 294–301PubMedCrossRefGoogle Scholar
  8. Wiernsperger, N., Sylvia, A.L. and Jöbsis, F.F. (1981) Incomplete transient ischemia: a non-destructive evaluation of in vivo cerebral metabolism and hemodynamics in rat brain. Stroke, 12: 864–868PubMedCrossRefGoogle Scholar
  9. Wray, S., Cope, M., Delpy, D.T., Wyatt, J.S. and Reynolds, E.O.R. (1988) Characterization of the near infrared absorption spectra of cytochrome aa3 and haemoglobin for the non-invasive monitoring of cerebral oxygenation. Biochim. Biophys. Acta, 933: 184–192PubMedCrossRefGoogle Scholar
  10. Wyatt, J.S., Cope, M., Delpy, D.T., Wray, S. and Reynolds, E.O.R. (1986) Quantification of cerebral oxygenation and haemodynamics in sick newborn infants by near infrared spectrophotometry. Lancet, II (1986): 1063–1066CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Osamu Hazeki
    • 1
  • Mamoru Tamura
    • 1
  1. 1.Biophysics Division Research Institute of Applied ElectricityHokkaido UniversitySapporo 060Japan

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