Advertisement

The Oxygen Dependence of the Energy State of Cardiac Tissue: 3 1P-NMR and Optical Measurement of Myoglobin in Perfused Rat Heart

  • H. Fukuda
  • H. Yasuda
  • S. Shimokawa
  • M. Tamura
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 248)

Abstract

The intregrity of cardiac function depends on the energy of the tissue where ATP is produced mainly by oxidative phosphorylation in the mitochondria. Thus, a critical oxygen concentration has been postulated below which function cannot be maintained. Araki et al. (1983) found oxygen consumption fell when cardiac tissue oxygen concentration fell below 10 wmM. However, energy state can be governed by factors other than oxygen concentration (Owen and Wilson, 1974). In the present study, we performed simultaneously measuring the energy state of isolated hearts, by 31P-NMR, and estimating the tissue oxygen concentration by optical spectroscopy. Similar in situ measurements in brain were reported previously (Tamura et al., 1988).

Keywords

Oxygen Concentration Cardiac Tissue Pyridine Nucleotide Lactate Release Oxygen Dependence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Araki, R., Tamura, M., and Yamazaki, I. 1983. The effect of intracellular oxygen concentration on lactate release, pyridine nucleotide reduction, and respiration rate in the cardiac tissue. Circ. Res., 53:448.PubMedGoogle Scholar
  2. Hoshi, Y., Hazeki, O., and Tamura, M. 1989. Oxygen characteristics of heme and copper in cytochrome oxidase. This issue.Google Scholar
  3. Owen, C.S., and Wilson, D.F. 1974. Control of respiration by the mitochondrial phosphorylation state. Arch. Biochem. Biophys., 161:581.PubMedCrossRefGoogle Scholar
  4. Tamura, M., Hazeki, 0., Nioka, S., Chance, B., and Smith, D.S. 1988. The simultaneous measurements of tissue oxygen concentration and energy state by near-infrared and nuclear magnetic resonance spectroscopy. Adv. Exp. Med. Biol., 222:359.PubMedGoogle Scholar
  5. Tamura, M., Oshino, N., Chance, B., and Silver, I. 1978. Optical measurements of intracellular oxygen concentration of rat heart in vitro. Arch. Biochem. Biophys., 191:8.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • H. Fukuda
    • 1
  • H. Yasuda
    • 1
  • S. Shimokawa
    • 2
  • M. Tamura
    • 3
  1. 1.Department of Cardiovascular MedicineSchool of MedicineJapan
  2. 2.NMR Laboratory, Hokkaido UniversityJapan
  3. 3.Research Institute of Applied ElectricitySapporoJapan

Personalised recommendations