31P NMR Studies of the Metabolic Status of Pig Hearts Preserved for Transplantation
Preservation of the donor heart during transport is of paramount importance for a successful transplantation procedure [1,2]. An increase in the preservation window from the current 4 — 5 hours to over 24 hours [3,4] would lead to more compatible cross-matches and wider geographical harvest of transplantable hearts. In spite of the large amount of research on the development of solutions and modalities, there is still much to be learned to achieve optimal organ preservation. Preservation solutions have evolved from simple high-potassium buffers to complex recipes [3,4].
KeywordsNuclear Magnetic Resonance Nuclear Magnetic Resonance Spectrum Nuclear Magnetic Resonance Spectroscopy Cardioplegic Solution Procaine Hydrochloride
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- R. Hetzer, H. Warnecke, and S. Schuler, (1986) The Donor Heart: Procurement, Selection, and Preservation. TranspL Proc. 28,27–30.Google Scholar
- C.J.A. Van Echteid, and T.J.C. Ruigrok, (1987) Nuclear Magnetic Resonance Spectroscopy in Experimental Cardiology in “Non-invasive Imaging of Cardiac Metabolism” Dordrecht Martinus Nijhoff Publ., 265–277.Google Scholar
- G.A. Elgavish, (1987) NMR Spectroscopy of the Intact Heart in “Biological Magnetic Resonance”, L.J. Berliner and J. Reuben, eds., Plenum Press, New York, 81–127.Google Scholar
- R. Deslauriers, W.J. Keon, S. Lareau, D. Moir, J.K. Saunders, I.C.P. Smith, K. Whitehead, and G.W. Mainwood, (1989) Preservation of High Energy Phosphates in Human Myocardium — A 31P NMR Study of the Effect of Temperature on Atrial Appendages. J. Thorac. Cardiovasc. Surg., (in press)Google Scholar
- G.W. Mainwood, S. Lareau, K. Whitehead, W.J. Keon, and R. Deslauriers, (1989) The Effects of Temperature and Buffer Concentration on the Metabolism of Human Atrial Appendages Measured by 31P NMR, Adv. in Exptl. Med. & Biol. — Oxygen Transport to Tissues, 12, K. Rakusan, G. Biro & T. Goldstick, eds., Plenum Press, N.Y. (in press).Google Scholar
- R.J. Connett, (1988) Analysis of Metabolic Control: New Insights Using Scaled Creatine Kinase Model, Am. J. Physiol, 254, R949–R959.Google Scholar
- L.D. Shorr, R.T. Thompson, G.D. Marsh, F.M. Keith, A.A. Driedger, and D.J. Magilligan, Improved Preservation of Isolated Rabbit Hearts Using Oxygenated Cardioplegic Solutions. A 31P NMRS Study, (private communication).Google Scholar
- H. Takami, H. Matsuda, S. Oumi, H. Watari, E. Furuya, K. Tagawa, and Y. Kawashima, (1987) Assessment of Possible Compartmentalization of Myocardial ATP and Characteristics of Depletion Under Ischemia Utilizing 31P NMR, Circulation, 76,247.Google Scholar