Oxygen Delivery and Performance in the Isolated, Perfused Rat Heart: Comparison of Perfusion with Aqueous and Perfluorocarbon-Containing Media
The isolated, Langendorff-perfused rat heart is used extensively in biomedical investigation. This preparation is generally perfused with aqueous buffers oxygenated with high-oxygen gas mixtures. In spite of high pO2, because of the poor solubility of oxygen in water, the oxygen content of such perfusates is quite low: generally not exceeding 1.5 ml/dl. Adequate oxygen delivery to the the myocytes can only be maintained under these conditions by nearly maximal coronary vasodilation.
KeywordsPerfusion Pressure Oxygen Delivery Pump Flow Coronary Perfusion Pressure Coronary Vascular Resistance
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