Oxygen Delivery and Performance in the Isolated, Perfused Rat Heart: Comparison of Perfusion with Aqueous and Perfluorocarbon-Containing Media

  • G. P. Biro
  • M. Masika
  • B. Korecky
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 248)


The isolated, Langendorff-perfused rat heart is used extensively in biomedical investigation. This preparation is generally perfused with aqueous buffers oxygenated with high-oxygen gas mixtures. In spite of high pO2, because of the poor solubility of oxygen in water, the oxygen content of such perfusates is quite low: generally not exceeding 1.5 ml/dl. Adequate oxygen delivery to the the myocytes can only be maintained under these conditions by nearly maximal coronary vasodilation.


Perfusion Pressure Oxygen Delivery Pump Flow Coronary Perfusion Pressure Coronary Vascular Resistance 
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  1. Beek, J. H. G. M. and Westerhof, N., 1989. Mismatch of regional myocardial metabolism and perfusion is a possible cause of tissue hypoxia. This issue.Google Scholar
  2. Biro, G. P. and Biais, P. 1987. Perfluorocarbon blood substitutes. C. R. C. Crit. Rev. Oncol. Hematol. 6:311.CrossRefGoogle Scholar
  3. Biro, G. P., 1983. Fluorocarbon and dextran hemodilution in myocardial ischemia. Can. J. Surg., 26:163.PubMedGoogle Scholar
  4. Deutschmann, W., Linder, E., and Deutschlander, N., 1984. Perfluorochemical perfusion of the isolated guinea pig heart. Pharmacology. 28:336.PubMedCrossRefGoogle Scholar
  5. Glogar, D. H., Kloner, R. A., Müller, J., DeBoer, L. W. V., and Braunwald, E., 1981. Fluorocarbons reduce ischemic damage after coronary occlusion. Science. 211:1439PubMedCrossRefGoogle Scholar
  6. Faithfull, N. S., Erdman, W., Fennema, M., and Kok, A. 1986. The effects of hemodilution with fluorocarbons or dextran on oxygen tensions in the acutely ischeamic myocardium. Brit. J. Anaesth. 58:1031PubMedCrossRefGoogle Scholar
  7. Faithfull, N. S. and Cain S. M., 1988. Cardiorespiratory consequences of fluorocarbon reactions in dogs. Biomat. Art. Cells, and Art. Organs 16:643.Google Scholar
  8. Nunn, G. R., Dance, G., Peters, J., and Cohn, L. H., 1983. Effect of fluorocarbon exchange transfusion on myocardial infarct size in dogs. Am. J. Cardiol., 52:203.PubMedCrossRefGoogle Scholar
  9. Rude, R. E., Glogar, D., Khuri, S. F., Kloner, R. F., Karaffa, S., Müller, J. E., Clark, L. C. jr., and Braunwald, E. 1982., Effects of intravenous fluorocarbons during and without oxygen enhancement on acute myocardial ischemic injury assessed by intramyocardial gas tensions. Am. Heart J., 103:986.PubMedCrossRefGoogle Scholar
  10. Segal, L. D. and Ensunsa, J. L., 1988. Albumin improves the stability and longevity of perfluorochemical-perfused hearts. Am. J. Physiol., 254:H1105.Google Scholar
  11. Segal, L. D. and Rendig, S. V., 1982. Isolated working rat heart perfusion with perfluorochemical emulsion. Fluosol-43. Am. J. Physiol., 242 (Heart Circ. Physiol. 11):H485.Google Scholar
  12. Tomera, J. F. and Geyer, R. P., 1982. Perfluorochemical perfusion of the rat heart: in vivo and in vitro. J. Molec. Cell. Cardiol., 14:573.CrossRefGoogle Scholar
  13. Vogel, H., Günther, H., Harrison, D. K., Anderer, W., Kessler, M., and Peter, K., 1989. Hemodilution and myocardial oxygen supply. The influence of Fluosol-DA. This issue.Google Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • G. P. Biro
    • 1
  • M. Masika
    • 1
  • B. Korecky
    • 1
  1. 1.Department of PhysiologyUniversity of OttawaOttawaCanada

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