Time Resolved Spectroscopic (TRS) and Continuous Wave Spectroscopic (CWS) Studies of Photon Migration in Human Arms and Limbs
The optical study of tissues began with the spectroscopic studies of Glenn Millikan in 1935 who proposed a “metabolic microscope” by which he would follow metabolic demand as expressed by the deoxygenation of myoglobin and hemoglobin in tissue. This was beautifully demonstrated in his studies of the cat soleus muscle during functional actvity (tetanic contraction and ischemia) (1). While the optical changes could be attributed to both hemoglobin and myoglobin, the demonstration of the effectiveness of the dual-wavelength technique using a differential detector and color filters was established in his pioneer studies. Applications to humans emerged in 1940 (2) with the “Millikan Oximeter” which was applied to the lobe of the ear, and using the same princiles, this presaged the popular “pulse oximeter” as applied to the human finger tip (3) Neither of these approaches presumed to provide intracranial homoglobinometry. Thus the results of Jöbsis-Vander Vleit and later Piantadosi on transcranial spectroscopy are noteworthy. They have evolved a much more sophisticated instrument which attempts to deconvolute cytochrome from hemoglobin and myoglobin changes in the exercising muscle (4,5). Such studies have been vexed by an unknown optical path requiring the need for either speculation or transfer of data from one model to another in abortive attempts to convert what has been termed justifiably a “trend indicator” to a quantitative spectroscopic technique.
KeywordsMagnetic Resonance Spectroscopy Tetanic Contraction Human Limb Time Resolve Spectroscopy High Oxygen Affinity
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- 2.G. A. Millikan, The oximeter, an instrument for measuring continuously the oxygen saturation of arterial blood in man. Rev. Sci. Instru. 13:W (1942).Google Scholar
- 7.G. Renault, M. Sinet, M. Muffat-Joly, J. Cornillault, J. and J. J. Pocidalo, In situ monitoring of myocardial metabolism by laser fluorimetry: Relevance of a test of local ischemia. Lasers and Surgery & Medicine 5:111, 1985.Google Scholar
- 9.B. Chance, J. S. Leigh, Jr. R. Greenfeld, H. Miyake, D. S. Smith and S. Nioka, Time Resolved Spectroscopy (TRS): A new approach to the spectroscopy of hemoglobin in Brain. New Eng. J.Med. Submitted 1988Google Scholar
- 10.B. Chance, S. Nioka, J. Kent, K. McCully, M. Fountain, R. Greenfeld, and G. Holtorn, Time Resolved Spectroscopy of Hemoglonbin and Myoglobin in Resting and Ischemic Muscle. Anal. Biochem. In press.Google Scholar
- 11.B. Chance (ed.) Photon Migration in Tissues. A Workshop Proceedings. W. DeGruyter and Co. in press, 1988Google Scholar
- 12.T. Tamura, O. Hazeki, S. Nioka, and B. Chance, B. In vivo study of tissue oxygen metabolism using optical and nuclear magnetic resonance spectroscopies. Ann. Rev. Physiol. 51: in press, 1988.Google Scholar
- 17.K. Kitagishi, L. Hao, and B. Chance, B. Heterogeneity Response of an Exercising Forearm as Studied by a Surface Coil Scan. 7th Annual Society of Magnetic Resonance in Medicine Mtg., San Francisco, CA.(Aug. 20–26), p. 339, 1988Google Scholar