Abstract
The measurement of oxygen in intact tissues has proven to be full of technical difficulties. For example, the direct determination of oxygen in tissue using microelectrodes is subject to error in the low range of oxygen pressures (< 1 uM) and is restricted to fixed sites of evaluation (Silver, 1984). Moreover, insertion of the electrode damages the tissue and disturbs the local milieu. Indirect qualitative measurements using surface fluorescence of NADH indicate the presence of anoxia in tissue (Barlow and Chance, 1976) but lacks internal standardization (Koretsky et al., 1987). Spectrophotometric measurements of hemoglobin (Kekonen et al, 1987) and myoglobin (Fabel and Lubbers, 1965; Tamura et al., 1978) are useful for oxygen pressures near their P50 but are subject to optical interference from changes in light scattering and from absorbtion by other chromophores. Alternatively, cryomicrospectrophotometry of myoglobin (Gayeski and Honig, 1986) can provide an accurate method for calculating intracellular oxygen pressure in some tissues. Because this method requires freezing of the tissue, it is impractical for monitoring changes in oxygenation during any length of time.
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References
Barlow, C.H. and Chance, B., 1976, Ischemic areas in perfused rat hearts: Measurement by NADH fluorescence photography. Science, 193: 909–910.
Fabel, H. and Lubbers, D.W., 1965, Measurement of reflection spectra of the beating heart. Biochem. Z., 341: 351–356.
Gayeski, T.E.J. and Honig, C.R., 1986, O2 gradients from sarcolemma to cell interior in red muscle at maximal VO2. Am. J. Physiol. 251: H789–H799.
Kekonen, E.M., Jauhonen, V.P. and Hassinen, I.E., 1987, Oxygen and substrate dependence of hepatic cellular respiration: Sinusoidal oxygen gradient and effects of ethanol in isolated perfused liver and hepatocytes. J.Cell Physiol., 133: 119–126.
Koretsky, A.P., Katz, L.A. and Balaban R.S., 1987, Determination of pyridine nucleotide fluorescence from the perfused heart using an internal standard. Am. J. Physiol., 253: H856–H862.
Rumsey, W.L., Vanderkooi, J.M., and Wilson, D.F., 1988, Imaging of phosphorescence: A novel method for measuring the oxygen distribution in perfused tissue. Science, in press.
Robiolio, M, Rumsey, W.L. and Wilson, D.F., 1988, Oxygen diffusion and mitochondrial respiration in neuroblastoma cells. Am. J. Physiol., in press.
Silver, I.A. 1984, Polarographic techniques of oxygen measurements. In: “Oxygen: An in-depth study of its Pathophysiology”, Gottlieb, S.F., Longmuir, I.S. and Totter, J.R. eds., Undersea Med. Soc. pub No. 62 (ws) 3-1-84, Bethesda, MD. pp 215–238.
Tamura, M. Oshino, N., Chance, B. and Silver, I.A., 1978, Optical measurements of intracellular oxygen concentration of rat heart in vitro. Arch. Biochem. Biophys. 191: 8–22.
Vanderkooi, J.M., Maniara, G., Green, T.J., and Wilson, D.F., 1987, An optical method for measurement of dioxygen based quenching of phosphorescence. J. Biol. Chem., 262: 5476–5482.
Vanderkooi, J.M., and Wilson, D.F., 1986, A new method for measuring oxygen in biological systems. In: Oxygen transport to tissue VIII, Longmuir, LA. ed., Plenum Press, New York, p. 189–193.
Wilson, D.F., Rumsey, W.L., Green, T.J., and Vanderkooi, J.M., 1988, The oxygen dependence of mitochondrial oxidative phosphorylation measured by a new optical method for measuring oxygen concentration. J. Biol. Chem., 263: 2712–2718.
Wilson, D.F., Vanderkooi, J.M., Green, T.J., Maniara, G., DeFeo, S.F. and Bloomgarden, D.C., 1987. A Versatile and Sensitive Method for Measuring Oxygen. In: “Oxygen Transport to Tissue IX”. Silver, I.A. and Silver, A., ed., Plenum Press, New York and London, (Adv. Exp. Med. Biol. 215, 71–77).
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© 1989 Plenum Press, New York
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Wilson, D.F., Rumsey, W.L., Vanderkooi, J.M. (1989). Oxygen Distribution in Isolated Perfused Liver Observed by Phosphorescence Imaging. In: Rakusan, K., Biro, G.P., Goldstick, T.K., Turek, Z. (eds) Oxygen Transport to Tissue XI. Advances in Experimental Medicine and Biology, vol 248. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5643-1_14
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DOI: https://doi.org/10.1007/978-1-4684-5643-1_14
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