A Comparative Study of Actin Filaments in Cochlear Hair Cells: Outer Hair Cells in the Apex of the Guinea Pig Cochlea Contain a Unique Ultrastructural Feature
Rhodamine-labelled phalloidin, a specific marker for filamentous actin, was used to describe the distribution of F-actin in the hair cells of the organs of Corti of guinea pigs, hooded rats, chinchillas and squirrel monkeys using surface preparations, cryosections, and isolated outer hair cells. The stereocilia and cuticular plates of all inner and outer hair cells were labelled, as were the lateral margins of outer hair cells of all species. In the outer hair cells of the apical turns of the guinea pig organ of Corti, an additional labelled structure was present in the apical cytoplasm of the cell. This infracuticular network of F-actin was never observed in outer hair cells of the basal turn of the guinea pig, nor in outer hair cells at any location in the organs of Corti of the other species examined.
KeywordsHair Cell Actin Filament Squirrel Monkey Outer Hair Cell Cochlear Hair Cell
Unable to display preview. Download preview PDF.
- Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, K. and Watson, J.D. (1983): Molecular Biology of the Cell, Garland Publishing, Inc. New York, 571–572.Google Scholar
- Ashmore, J.F. (1986): The cellular physiology of isolated outer hair cells: implications for cochlear frequency selectivity. In: Auditory Frequency Selectivity. B.C.J. Moore and R.J. Patterson, ed. New York, Plenum Press, pp. 103–108.Google Scholar
- Dallos, P. (1985b): The role of outer hair cells in cochlear function. In: Contemporary Sensory Neurobiology, M.J. Correia and A.A. Perachio, eds. Alan Liss, New York, pp. 207–230.Google Scholar
- Drenkhahn, D., Schafer, T. and Prinz, M. (1985): Actin, myosin and associated proteins in the vertebrate auditory and vestibular organs: Immunocytochemical and biochemical studies. In: Auditory Biochemistry, D. G. Drescher, ed. Thomas, Springfield, Il. pp. 310–316.Google Scholar
- Pujol, R. and Gil-Loyzaga, P. (1987): Distribution of the two types of afferent dendrite in the developing cochlea: a kainic acid study. Abstr. Assoc. Res. Otolaryngol. 10, p. 122.Google Scholar
- Russell, I.J. and Cody, A.R. (1985): Transduction in cochlear hair cells. In: Peripheral Auditory Mechanisms: J.B. Allen, J.L. Hall, A. Hubbard, S.T. Neely and A. Tubis, eds. Springer-Verlag, New York, pp. 349–360.Google Scholar
- Zenner, H.P. (1980): Cytoskeletal and muscle like elements in cochlear hair cells. Arch. Otorhinolaryngol. 230, 82–92.Google Scholar
- Zenner, H.P. and Drenkhahn, D. (1986): Active motile events in guinea pig outer hair cells. Abstr. Assoc. Res. Otolarygol. 9, 1.Google Scholar