Purification of Polyamine Oxidase from Maize Seedlings by Immunoadsorbent Column

  • Rodolfo Federico
  • Chiara Alisi
  • Alessandra Cona
  • Riccardo Angelini
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 250)


Plant polyamine oxidases (PAOs) are typical of the monocotyledones and are responsible for the terminal catabolism of polyamines, producing H2O2, diaminopropane and Δ′-pyrroline (from spermidine) or diazabicyclononane (from spermine)(Smith, 1985a; Smith et al., 1986). These enzymes are quite distinct from diamine oxidases (DAOs) which occur in the dicotyledones (Smith, 1985a; Rinaldi et al., 1986) the latter containing pyrroloquinolinequinone (Glatz et al., 1987; Suzuki 1987) as the cofactor while PAOs are flavoproteins (Smith, 1983). In general DAOs have a broader substrate specificity oxidizing diamines but also spermine and spermidine (Chaudhuri and Gosh, 1984).


Water Hyacinth Maize Seedling Diamine Oxidase Polyamine Oxidase Jerusalem Artichoke Tuber 
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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Rodolfo Federico
    • 1
  • Chiara Alisi
    • 1
  • Alessandra Cona
    • 1
  • Riccardo Angelini
    • 1
  1. 1.Dipartimento di Biologia VegetaleUniversità degli Studi di Roma “La Sapienza”RomeItaly

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