Effect of Spermine on Transfer RNA and Transfer RNA-Ribosome Interactions
Translation of genetic message requires a coordianted interplay of more than a hundred kinds of macromolecules. Besides the ribosomes, which are multicomponent ribonucleoprotein particles by their own nature, molecules of transfer RNA, aminoacyl-tRNA synthetases, soluble protein factors and mRNA are involved in the process. Ribosomes provide an unspecific stage for the codon-anticodon interaction and catalyze the peptide bond formation. Molecules of tRNA play a crucial and highly specific role in the over-all process: they are recognized by specific aminoacyl-tRNA synthetases to be charged with their cognate amino acids; the resulting aminoacyl-tRNAs are then brought, in the form of ternary complexes with GTP and the elongation factor Tu, to the decoding or A site of the ribo-some. There they are screened for the proper codon-anticodon matching and only the correct aminoacyl-tRNA is allowed to enter the transpeptidation reaction. The resulting peptidyl-tRNA is then translocated to the peptidyl or P site to serve as the peptidyl donor in the next round of translation. The process in its simplified form is shown in Figure 1.
KeywordsTernary Complex Spin Label Anticodon Loop tRNA Structure Anticodon Stem
Unable to display preview. Download preview PDF.
- 7.M. Plohl and Ž. Kućan, Effects of Spermine and Magnesium Ions on the Aminoacylation of Yeast tRNATyr, Biochemie, in press (1988).Google Scholar
- 23.Ž. Kućan and R.W. Chambers, Purification of Tyrosine-tRNA Ligase from Saccharomyces cerevisiae S288C, J. Biochem. (Tokyo), 73: 811 (1973).Google Scholar
- 25.S. Pestka, Inhibitors of Protein Synthesis, in “Molecular Mechanism of Protein Synthesis”, H. Weissbac and S. Pestka, eds., Academic Press, New York (1977).Google Scholar
- 29.Ž. Kućan, On the Role of Spermine in Protein Synthesis, in “The Roots of Modern Biochemistry”, H. Kleinkauf, H. von Döhren, and L. Jaenicke, eds. Walter de Gruyter, Berlin (1988).Google Scholar