Uptake of Polyamines by Human Lymphocytes and Their Effect on Lactate Formation from Glucose
The existence of a polyamine transport system has been demonstrated in both prokaryotes and eukaryotes. However, although its physiological significance has been suggested by several findings1 the system is poorly Known. It has been usually studied in tumor cells, in the aim of modifying the intracellular polyamine concentration, but seldom in normal cells. According to Seppänen et al.2 exogenous polyamines are taken up by mammalian cells extremely slowly, with only negligible changes in their intracellular pools. However, erythrocytes take up polyamines and transport them3, 4. The ability of other blood cells, such as lymphocytes, to do so is not known. These cells, in contrast with erythrocytes, are able to synthesize polyamines, but it is not Known whether they are also able to metabolize exogenous polyamines. According to Kay and Lindsay5, stimulated lymphocytes take up putrescine, but only a very small proportion of the labelled compound enters the cells within 3 hours. No data are available for spermidine and spermine. Moreover, it is not known whether polyamines accumulate inside these cells, and whether they have an effect on the cellular metabolism. This would be important in vivo, as polyamine concentration is modified in blood in several conditions.
KeywordsEhrlich Ascites Carcinoma Dansyl Chloride Mouse Neuroblastoma Cell Lactate Formation Polyamine Concentration
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