Biosynthesis of Hypusine in eIF-4D Precursors
An unusual basic amino acid hypusine was discovered in 1971 in extracts of bovine brain by Shiba and coworkers, who determined its chemical structure as Nε -(4-amino-2-hydroxybutyl)lysine(1). Its distribution in various animal tissues as the free amino acid (2) and as a component of protein (3) was subsequently reported. Initial attempts to isolate any specific protein that contained hypusine were unsucessful, however (3). Ten years after the discovery of hypusine, we observed radiolabeling of a single cellular protein (Mr 183000, pI 5.3) upon incubation of human peripheral lymphocytes with [1, 8- 3H] spermidine or [2, 3- 3H] putrescine in the presence of the mitogen, phytohemagglutinin and identified the polyamine-derived radioactive component of this protein as hypusine (4).
KeywordsProtein Substrate Ammonium Sulfate Fraction Bold Face Type Human Peripheral Lymphocyte Break Arrow
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