Purification and Structural Characterization of In Vitro Synthesized (γ-Glutamyl) Spermidine Conjugates of a Major Protein Secreted from the Rat Seminal Vesicles

  • R. Porta
  • C. Esposito
  • S. Metafora
  • A. Malorni
  • P. Pucci
  • G. Marino
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 250)


Very little is known about the physiological meaning of spermidine (Spd) and spermine (Spm) occurrence in mammal seminal plasma where polyamines are secreted in large amounts by the prostate (1–3). One postulated role for semen polyamines is to serve as acyl acceptor substrates for transglutaminase (TGase; EC In fact, it has been demonstrated that rat seminal clot proteins have covalently bound polyamines and upon proteolytic digestion released (γ-glutamyl)polyamine derivatives (4). MDreover, N-mono- and N, N-bis-(γ-glutamyl)polyamine derivatives were found after proteolysis of rat vesicular secretion proteins that have been incubated with coagulating gland extracts (5). Some of us have recently shown the ability of one of the major proteins secreted from the rat seminal vesicle epithelium to covalently bind radioactive Spd in the presence of either purified guinea pig liver TGase or crude secretory fluid produced by the rat coagulating gland (6). Such a protein, named SV-IV, is the fourth major band observed on the SDS-PAGE pattern of the proteins present in the rat seminal vesicle secretion. The sequence of its 90 amino acids (Mr= 9, 758) and the main features of the gene coding for it are known (6–9). We have demonstrated that SV-IV possesses immunosuppressive and anti-inflanmatory activities (6, 10, 11). Microgram amounts of SV-IV are able to inhibit both the mitogen-induced lymphocyte blastogenesis and the mixed lymphocyte reaction; the observed anti-inflaitmatory activity, comparable to that of dexamethasone, seems to be due to the block of arachidonic acid cascade at level of the enzyme phospholipase A2.


Seminal Plasma Major Protein Proteolytic Digest Arachidonic Acid Cascade Partial Spectrum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    O. Rosenheim, The isolation of spermine phosphate from semen and testis, Biochem. J. 18: 1253 (1924).PubMedGoogle Scholar
  2. 2.
    H. G. Williams-Ashnan, A. E. Pegg and D. H. Lockwood, Mechanisms and regulation of polyamine and putrescine biosynthesis in male genital glands and other tissues of mammals, Adv. Enzyme Regul. 7: 291 (1969).CrossRefGoogle Scholar
  3. 3.
    H. G. Williams-Ashman, A. Corti and A.R. Sheth, Formation and functions of aliphatic polyamines in the prostate gland and its secretions, in “Normal and Abnormal Growth of the Prostate”, Goland M., ed., Thomas, Springfield (1975).Google Scholar
  4. 4.
    H. G. Williams-Ashman and Z.N. Canellakis, Transglutaminase-mediated covalent attachment of polyamines to proteins: mechanisms and potential physiological significance, Physiol. Chem. Phys. 12: 457 (1980).PubMedGoogle Scholar
  5. 5.
    J. E. Folk, M.H. Park, S.I. Chung, J. Schrode, E.P. Lester and H.L. Cooper, Polyamines as physiological substrates for transglutaminase, J. Biol. Chem. 255: 3695 (1980).PubMedGoogle Scholar
  6. 6.
    R. Porta, C. Esposito, P. Persico, G. Peluso and S. Metafora, Transglutaminase-catalyzed crosslinking of an immunosuppressive and anti-inflanmatory protein secreted from the rat seminal vesicles, Adv. Exp. Med. & Biol. 231: 153 (1988).Google Scholar
  7. 7.
    Y. E. Pan and S. S. Li, Structure of secretory protein IV from rat seminal vesicles, Int. J. Pept. Prot. Res. 20: 177 (1978).CrossRefGoogle Scholar
  8. 8.
    S. J. Higgins and J. M. Burchell, Effects of testosterone on messenger ribonucleic acid and protein synthesis in rat seminal vesicle, Biochem. J. 174: 543 (1978).PubMedGoogle Scholar
  9. 9.
    M. C. Ostrowski, M. K. Kistler and W. S. Kistler, Purification and cell-free synthesis of a major protein from rat seminal vesicle secretion. A potential marker for androgen action, J. Biol. Chem. 254: 383 (1979).PubMedGoogle Scholar
  10. 10.
    S. Metafora, G. Peluso, G. Ravagnan, V. Gentile, A. Fusco, R. Porta, Factors modulating immunocompatibility of spermatozoa: role of transglutaminase and SV-IV, one of the major proteins secreted from the rat seminal vesicle epithelium, in: “Morphological Basis of Human Reproductive function”, Spera G. and de Kretser D.M., eds., Plenum Press, New York and London (1987).Google Scholar
  11. 11.
    S. Metafora, G. Peluso, G. Ravagnan, P. Persico, C. Esposito and R. Porta, Immunosuppressive and anti-inflammatory properties of the major protein secreted from the rat seminal vesicle epithelium, Biochem. Pharmacol., in press.Google Scholar
  12. 12.
    A. Dell and M. Panico, Fast Atom Bombardment mass spectrometry of biomolecules, in: “Mass Spectrometry in Biomedical Research”, S. Gaskell, ed., Wiley, Chichester (1986).Google Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • R. Porta
    • 1
  • C. Esposito
    • 1
  • S. Metafora
    • 3
  • A. Malorni
    • 4
  • P. Pucci
    • 2
  • G. Marino
    • 2
  1. 1.Departments of Biochemistry and BiophysicsUniversity of NaplesNaplesItaly
  2. 2.Departments of Organic and Biological ChemistryUniversity of NaplesNaplesItaly
  3. 3.International Institute of Genetics and BiophysicsNaplesItaly
  4. 4.Institute of Chemistry of Molecules of Biological InterestCNRArco Felice, NaplesItaly

Personalised recommendations